PMID- 22936024
OWN - NLM
STAT- MEDLINE
DCOM- 20130212
LR  - 20240210
IS  - 1521-4141 (Electronic)
IS  - 0014-2980 (Linking)
VI  - 42
IP  - 12
DP  - 2012 Dec
TI  - CD64 distinguishes macrophages from dendritic cells in the gut and reveals the 
      Th1-inducing role of mesenteric lymph node macrophages during colitis.
PG  - 3150-66
LID - 10.1002/eji.201242847 [doi]
AB  - Dendritic cells (DCs) and monocyte-derived macrophages (MPhis) are key components 
      of intestinal immunity. However, the lack of surface markers differentiating MPhis 
      from DCs has hampered understanding of their respective functions. Here, we 
      demonstrate that, using CD64 expression, MPhis can be distinguished from DCs in the 
      intestine of both mice and humans. On that basis, we revisit the phenotype of 
      intestinal DCs in the absence of contaminating MPhis and we delineate a 
      developmental pathway in the healthy intestine that leads from newly extravasated 
      Ly-6C(hi) monocytes to intestinal MPhis. We determine how inflammation impacts this 
      pathway and show that T cell-mediated colitis is associated with massive 
      recruitment of monocytes to the intestine and the mesenteric lymph node (MLN). 
      There, these monocytes differentiate into inflammatory MPhis endowed with 
      phagocytic activity and the ability to produce inducible nitric oxide synthase. 
      In the MLNs, inflammatory MPhis are located in the T-cell zone and trigger the 
      induction of proinflammatory T cells. Finally, T cell-mediated colitis develops 
      irrespective of intestinal DC migration, an unexpected finding supporting an 
      important role for MLN-resident inflammatory MPhis in the etiology of T 
      cell-mediated colitis.
CI  - (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Tamoutounour, Samira
AU  - Tamoutounour S
AD  - Centre d'Immunologie de Marseille-Luminy, Aix Marseille Universite, Marseille, 
      France.
FAU - Henri, Sandrine
AU  - Henri S
FAU - Lelouard, Hugues
AU  - Lelouard H
FAU - de Bovis, Beatrice
AU  - de Bovis B
FAU - de Haar, Colin
AU  - de Haar C
FAU - van der Woude, C Janneke
AU  - van der Woude CJ
FAU - Woltman, Andrea M
AU  - Woltman AM
FAU - Reyal, Yasmin
AU  - Reyal Y
FAU - Bonnet, Dominique
AU  - Bonnet D
FAU - Sichien, Dorine
AU  - Sichien D
FAU - Bain, Calum C
AU  - Bain CC
FAU - Mowat, Allan McI
AU  - Mowat AM
FAU - Reis e Sousa, Caetano
AU  - Reis e Sousa C
FAU - Poulin, Lionel F
AU  - Poulin LF
FAU - Malissen, Bernard
AU  - Malissen B
FAU - Guilliams, Martin
AU  - Guilliams M
LA  - eng
GR  - 15689/CRUK_/Cancer Research UK/United Kingdom
GR  - MRC_/Medical Research Council/United Kingdom
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121017
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antigens, Ly)
RN  - 0 (Ly-6C antigen, mouse)
RN  - 0 (Receptors, IgG)
SB  - IM
CIN - Eur J Immunol. 2012 Dec;42(12):3136-40. PMID: 23255010
MH  - Animals
MH  - Antigens, Ly/immunology
MH  - Cell Differentiation/immunology
MH  - Colitis/*immunology/pathology
MH  - Dendritic Cells/*immunology/pathology
MH  - Humans
MH  - Immunity, Mucosal
MH  - Intestinal Mucosa/*immunology/pathology
MH  - Lymph Nodes/*immunology/pathology
MH  - Macrophages/*immunology/pathology
MH  - Mesentery/*immunology/pathology
MH  - Mice
MH  - Mice, Knockout
MH  - Monocytes/immunology/pathology
MH  - Receptors, IgG/*immunology
MH  - Th1 Cells/*immunology/pathology
EDAT- 2012/09/01 06:00
MHDA- 2013/02/13 06:00
CRDT- 2012/09/01 06:00
PHST- 2012/07/20 00:00 [received]
PHST- 2012/08/26 00:00 [revised]
PHST- 2012/08/28 00:00 [accepted]
PHST- 2012/09/01 06:00 [entrez]
PHST- 2012/09/01 06:00 [pubmed]
PHST- 2013/02/13 06:00 [medline]
AID - 10.1002/eji.201242847 [doi]
PST - ppublish
SO  - Eur J Immunol. 2012 Dec;42(12):3150-66. doi: 10.1002/eji.201242847. Epub 2012 Oct 
      17.