PMID- 22936120
OWN - NLM
STAT- MEDLINE
DCOM- 20130611
LR  - 20211021
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 37
IP  - 12
DP  - 2012 Dec
TI  - Quercetin attenuates cell apoptosis in focal cerebral ischemia rat brain via 
      activation of BDNF-TrkB-PI3K/Akt signaling pathway.
PG  - 2777-86
LID - 10.1007/s11064-012-0871-5 [doi]
AB  - Many studies have demonstrated that apoptosis play an important role in cerebral 
      ischemic pathogenesis and may represent a target for treatment. Neuroprotective 
      effect of quercetin has been shown in a variety of brain injury models including 
      ischemia/reperfusion. It is not clear whether BDNF-TrkB-PI3K/Akt signaling 
      pathway mediates the neuroprotection of quercetin, though there has been some 
      reports on the quercetin increased brain-derived neurotrophic factor (BDNF) level 
      in brain injury models. We therefore first examined the neurological function, 
      infarct volume and cell apoptosis in quercetin treated middle cerebral artery 
      occlusion (MCAO) rats. Then the protein expression of BDNF, cleaved caspase-3 and 
      p-Akt were evaluated in either the absence or presence of PI3K inhibitor 
      (LY294002) or tropomyosin receptor kinase B (TrkB) receptor antagonist (K252a) by 
      immunohistochemistry staining and western blotting. Quercetin significantly 
      improved neurological function, while it decreased the infarct volume and the 
      number of TdT mediated dUTP nick end labeling positive cells in MCAO rats. The 
      protein expression of BDNF, TrkB and p-Akt also increased in the quercetin 
      treated rats. However, treatment with LY294002 or K252a reversed the 
      quercetin-induced increase of BDNF and p-Akt proteins and decrease of cleaved 
      caspase-3 protein in focal cerebral ischemia rats. These results demonstrate that 
      quercetin can decrease cell apoptosis in the focal cerebral ischemia rat brain 
      and the mechanism may be related to the activation of BDNF-TrkB-PI3K/Akt 
      signaling pathway.
FAU - Yao, Rui-Qin
AU  - Yao RQ
AD  - Department of Neurobiology, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou, 
      221002 Jiangsu, People's Republic of China. wenxi_yao@163.com
FAU - Qi, Da-Shi
AU  - Qi DS
FAU - Yu, Hong-Li
AU  - Yu HL
FAU - Liu, Jing
AU  - Liu J
FAU - Yang, Li-Hua
AU  - Yang LH
FAU - Wu, Xiu-Xiang
AU  - Wu XX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120831
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 9IKM0I5T1E (Quercetin)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Blotting, Western
MH  - Brain Ischemia/enzymology/metabolism/*pathology
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Quercetin/*pharmacology
MH  - Rats
MH  - Receptor, trkB/*metabolism
MH  - Signal Transduction/*drug effects
EDAT- 2012/09/01 06:00
MHDA- 2013/06/12 06:00
CRDT- 2012/09/01 06:00
PHST- 2012/06/03 00:00 [received]
PHST- 2012/08/11 00:00 [accepted]
PHST- 2012/07/31 00:00 [revised]
PHST- 2012/09/01 06:00 [entrez]
PHST- 2012/09/01 06:00 [pubmed]
PHST- 2013/06/12 06:00 [medline]
AID - 10.1007/s11064-012-0871-5 [doi]
PST - ppublish
SO  - Neurochem Res. 2012 Dec;37(12):2777-86. doi: 10.1007/s11064-012-0871-5. Epub 2012 
      Aug 31.