PMID- 22936497
OWN - NLM
STAT- MEDLINE
DCOM- 20130524
LR  - 20211021
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 45
IP  - 3
DP  - 2012 Dec
TI  - Use of reverse genetics to develop a novel marker porcine reproductive and 
      respiratory syndrome virus.
PG  - 548-55
LID - 10.1007/s11262-012-0812-z [doi]
AB  - Nucleocapsid (N) protein of porcine reproductive and respiratory syndrome virus 
      (PRRSV) is the most abundant viral structural protein with high immunogenicity. 
      Previously, the nonessential sequences for virus infectivity were identified at 
      both N and C terminal ends of N protein. Here, by means of reverse genetics, a 
      marker virus (v7APMa) was generated with a mutant N protein that differs from the 
      wild-type strains (vAPRRS, type 2 PRRSV). v7APMa shows stable inheritance in cell 
      culture and the virologic characteristics of the marker virus in vitro showed 
      that v7APMa replicates well as its parental strain. In the pig model, the v7APMa 
      marker virus induced a similar level of anti-N protein antibodies and robust 
      antibodies against the marker peptide, from 14 days post infection. In addition, 
      a peptide-based ELISA was developed to detect the specific antibodies for the 
      introduced 7APMa peptide. This approach, using a rationally designed marker 
      virus, provides a new potential mutant basis for further development of PRRSV 
      novel vaccines.
FAU - Lin, Tao
AU  - Lin T
AD  - College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Weigang 
      Street, Xuanwu District, Nanjing, People's Republic of China.
FAU - Li, Xiangrui
AU  - Li X
FAU - Yao, Huochun
AU  - Yao H
FAU - Wei, Zuzhang
AU  - Wei Z
FAU - Tan, Feifei
AU  - Tan F
FAU - Liu, Runxia
AU  - Liu R
FAU - Sun, Lichang
AU  - Sun L
FAU - Zhang, Rong
AU  - Zhang R
FAU - Li, Wenliang
AU  - Li W
FAU - Lu, Jiaqi
AU  - Lu J
FAU - Tong, Guangzhi
AU  - Tong G
FAU - Yuan, Shishan
AU  - Yuan S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120831
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (Antibodies, Viral)
RN  - 0 (Biomarkers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Antibody Formation
MH  - Biomarkers/metabolism
MH  - Cell Line
MH  - DNA, Complementary/genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunity, Humoral
MH  - Models, Animal
MH  - Molecular Sequence Data
MH  - Mutagenesis
MH  - Nucleocapsid Proteins/*genetics/immunology
MH  - Open Reading Frames
MH  - Peptides/genetics/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*genetics/immunology/pathogenicity/physiology
MH  - Reverse Genetics/*methods
MH  - Swine
MH  - Transfection
MH  - Viral Plaque Assay
MH  - Virus Replication
EDAT- 2012/09/01 06:00
MHDA- 2013/05/28 06:00
CRDT- 2012/09/01 06:00
PHST- 2012/03/03 00:00 [received]
PHST- 2012/08/22 00:00 [accepted]
PHST- 2012/09/01 06:00 [entrez]
PHST- 2012/09/01 06:00 [pubmed]
PHST- 2013/05/28 06:00 [medline]
AID - 10.1007/s11262-012-0812-z [doi]
PST - ppublish
SO  - Virus Genes. 2012 Dec;45(3):548-55. doi: 10.1007/s11262-012-0812-z. Epub 2012 Aug 
      31.