PMID- 22938542
OWN - NLM
STAT- MEDLINE
DCOM- 20130125
LR  - 20211021
IS  - 1840-4812 (Electronic)
IS  - 1512-8601 (Print)
IS  - 1512-8601 (Linking)
VI  - 12
IP  - 3
DP  - 2012 Aug
TI  - Hypoxia induces voltage-gated K+ (Kv) channel expression in pulmonary arterial 
      smooth muscle cells through hypoxia-inducible factor-1 (HIF-1).
PG  - 158-63
AB  - Hypoxia-inducible factor-1 (HIF-1) regulates the expression of hypoxia-inducible 
      genes by binding erythropoietin (EPO) enhancer fragments. Of these genes, HIF-1 
      upregulates voltage-gated K+1.2 channels (Kv1.2) in rat PC12 cells. Whether HIF-1 
      regulates hypoxia-induced Kv channel expression in cultured pulmonary artery 
      smooth muscle cells (PASMCs), however, has not been determined. In this study, we 
      investigated the effects of hypoxia on the expression of Kv1.2 Kv1.5, Kv2.1, and 
      Kv9.3 channels in PASMCs and examined the direct role of HIF-1 by transfecting 
      either wild type or mutant EPO enhancer fragments. Our results showed that 18 h 
      exposure to hypoxia significantly increased the expression of Kv1.2, Kv1.5, 
      Kv2.1, and Kv9.3; and this hypoxia-induced upregulation was completely inhibited 
      after transfection with the wild type but not mutant EPO enhancer fragment. These 
      results indicate that HIF-1 regulates hypoxia-stimulated induction of Kv1.2 
      Kv1.5, Kv2.1, and Kv9.3 channels in cultured PASMCs.
FAU - Dong, Qian
AU  - Dong Q
AD  - Ion Channelopathy Research Center, Institute of Cardiovascular Diseases, Union 
      Hospital, Tongji Medical College, Huazhong University of Science and Technology, 
      Wuhan, China.
FAU - Zhao, Ning
AU  - Zhao N
FAU - Xia, Cheng-Kun
AU  - Xia CK
FAU - Du, Li-li
AU  - Du LL
FAU - Fu, Xiao-Xing
AU  - Fu XX
FAU - Du, Yi-Mei
AU  - Du YM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Bosnia and Herzegovina
TA  - Bosn J Basic Med Sci
JT  - Bosnian journal of basic medical sciences
JID - 101200947
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Kcnb1 protein, rat)
RN  - 0 (Kv1.2 Potassium Channel)
RN  - 0 (Kv1.5 Potassium Channel)
RN  - 0 (Mutant Proteins)
RN  - 0 (Potassium Channels, Voltage-Gated)
RN  - 0 (RNA, Messenger)
RN  - 0 (Shab Potassium Channels)
RN  - 11096-26-7 (Erythropoietin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - DNA/genetics
MH  - Enhancer Elements, Genetic
MH  - Erythropoietin/genetics
MH  - Gene Expression
MH  - Hypoxia/*genetics/*metabolism
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
MH  - Kv1.2 Potassium Channel/genetics
MH  - Kv1.5 Potassium Channel/genetics
MH  - Muscle, Smooth, Vascular/metabolism
MH  - Mutant Proteins/genetics
MH  - PC12 Cells
MH  - Potassium Channels, Voltage-Gated/*genetics
MH  - Pulmonary Artery/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Shab Potassium Channels/genetics
MH  - Transfection
PMC - PMC4362424
EDAT- 2012/09/04 06:00
MHDA- 2013/01/26 06:00
PMCR- 2012/08/01
CRDT- 2012/09/04 06:00
PHST- 2012/09/04 06:00 [entrez]
PHST- 2012/09/04 06:00 [pubmed]
PHST- 2013/01/26 06:00 [medline]
PHST- 2012/08/01 00:00 [pmc-release]
AID - BJBMS-12-158 [pii]
AID - 10.17305/bjbms.2012.2463 [doi]
PST - ppublish
SO  - Bosn J Basic Med Sci. 2012 Aug;12(3):158-63. doi: 10.17305/bjbms.2012.2463.