PMID- 22939380
OWN - NLM
STAT- MEDLINE
DCOM- 20121226
LR  - 20120914
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1258
DP  - 2012 Oct 5
TI  - Ultra high performance liquid chromatography-electrospray ionization-tandem mass 
      spectrometry screening method for direct analysis of designer drugs, "spice" and 
      stimulants in oral fluid.
PG  - 37-42
LID - S0021-9673(12)01219-8 [pii]
LID - 10.1016/j.chroma.2012.07.098 [doi]
AB  - An ultra high performance liquid chromatography-electrospray ionization-tandem 
      mass spectrometry (UHPLC-ESI-MS/MS) screening method for the direct analysis in 
      oral fluid (OF) of 24 drugs, including new synthetic cannabinoids and so-called 
      "smart" designer drugs, in a single chromatographic run was set up. 
      Benzylpiperazine, methylone, 5,6-methylenedioxy-2-aminoindane (MDAI), 
      fenproporex, 4-fluoroamphetamine (4-FA), 4-methyl-N-ethylcathinone (4-MEC), 
      4-methylamphetamine (4-MA), methylbenzodioxolylbutanamine (MBDB), mephedrone, 
      methylthioamphetamine (MTA), methylenedioxypyrovalerone (MDPV), mefenorex, 
      nabilone, furfenorex, clobenzorex, JWH-200, AM 694, JWH-250, JWH-073, JWH-018, 
      JWH-019, JWH-122, HU 210 and CP 47497 were determined in a chromatographic run of 
      9 min only with no sample pre-treatment, after addition of ISs and dilution in 
      mobile phase A. This method is designed to be applied to 250 muL of OF sample, 
      anyway is suitable to be used on smaller volumes (till 100 muL). LODs vary from 
      1ng/mL to 20 ng/mL. No interfering peaks were observed due to similar analytes, 
      common therapeutic drugs or endogenous compounds. Matrix effect, although present 
      especially for mephedrone, is acceptable, allowing the detection of the compounds 
      at the LODs described. The developed method was applied on 400 real OF samples 
      from on-site tests performed by police officers.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Strano-Rossi, Sabina
AU  - Strano-Rossi S
AD  - Institute of Legal Medicine, Catholic University of Sacred Heart, L.go F. Vito 1, 
      00168 Rome, Italy. sabina.stranorossi@rm.unicatt.it
FAU - Anzillotti, Luca
AU  - Anzillotti L
FAU - Castrignano, Erika
AU  - Castrignano E
FAU - Romolo, Francesco Saverio
AU  - Romolo FS
FAU - Chiarotti, Marcello
AU  - Chiarotti M
LA  - eng
PT  - Journal Article
DEP - 20120813
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Designer Drugs)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Designer Drugs/*analysis
MH  - Humans
MH  - Limit of Detection
MH  - Linear Models
MH  - Reproducibility of Results
MH  - Saliva/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2012/09/04 06:00
MHDA- 2012/12/27 06:00
CRDT- 2012/09/04 06:00
PHST- 2012/06/19 00:00 [received]
PHST- 2012/07/30 00:00 [revised]
PHST- 2012/07/31 00:00 [accepted]
PHST- 2012/09/04 06:00 [entrez]
PHST- 2012/09/04 06:00 [pubmed]
PHST- 2012/12/27 06:00 [medline]
AID - S0021-9673(12)01219-8 [pii]
AID - 10.1016/j.chroma.2012.07.098 [doi]
PST - ppublish
SO  - J Chromatogr A. 2012 Oct 5;1258:37-42. doi: 10.1016/j.chroma.2012.07.098. Epub 
      2012 Aug 13.