PMID- 22941598
OWN - NLM
STAT- MEDLINE
DCOM- 20130110
LR  - 20131121
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 920
DP  - 2012
TI  - Detection of DNA damage by comet fluorescence in situ hybridization.
PG  - 91-100
LID - 10.1007/978-1-61779-998-3_7 [doi]
AB  - Comet fluorescence in situ hybridization (Comet-FISH) is a useful method to 
      detect overall and region-specific DNA damage in individual cells. Two 
      well-established methods are combined, the Comet assay (single cell gel 
      electrophoresis) and fluorescence in situ hybridization (FISH). The Comet assay 
      is the method of choice for the detection of DNA damage. With the alkaline 
      version the influence of specific substances such as water pollutants or 
      ingredients of food on individual cells can be easily measured. The Comet assay 
      involves the embedding of cells in agarose on microscopic slides, lysis of cells, 
      and separation of DNA via electrophoresis. Damaged DNA migrates from the nucleus 
      (head of the comet) forming a tail. The percentage of DNA in the tail correlates 
      with the degree of DNA strand breaks (DNA damage). The combination of FISH with 
      the Comet assay uses labeled probes which hybridize specifically to selected DNA 
      sequences. This allows the detection of specific DNA damage or repair capacity in 
      single cells. Here we present exemplarily the Comet-FISH method by detection of 
      DNA damage using hydrogen peroxide as a genotoxic model substrate.
FAU - Schlormann, Wiebke
AU  - Schlormann W
AD  - Department of Nutritional Physiology, Institute for Nutrition, 
      Friedrich-Schiller-University Jena, Jena, Germany.
FAU - Glei, Michael
AU  - Glei M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Mutagens)
RN  - NQ1SX9LNAU (Digoxigenin)
SB  - IM
MH  - Comet Assay/*methods
MH  - *DNA Damage
MH  - Digoxigenin/metabolism
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mutagens/toxicity
MH  - Nucleic Acid Hybridization
MH  - Staining and Labeling
EDAT- 2012/09/04 06:00
MHDA- 2013/01/11 06:00
CRDT- 2012/09/04 06:00
PHST- 2012/09/04 06:00 [entrez]
PHST- 2012/09/04 06:00 [pubmed]
PHST- 2013/01/11 06:00 [medline]
AID - 10.1007/978-1-61779-998-3_7 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2012;920:91-100. doi: 10.1007/978-1-61779-998-3_7.