PMID- 22943528
OWN - NLM
STAT- MEDLINE
DCOM- 20131017
LR  - 20120904
IS  - 1740-0929 (Electronic)
IS  - 1344-3941 (Linking)
VI  - 83
IP  - 9
DP  - 2012 Sep
TI  - New strategies of boar sperm cryopreservation: development of novel freezing and 
      thawing methods with a focus on the roles of seminal plasma.
PG  - 623-9
LID - 10.1111/j.1740-0929.2012.01034.x [doi]
AB  - Cryopreservation of boar spermatozoa offers an effective means of long-term 
      storage of important genetic material. Many researchers have investigated how to 
      improve reproductive performance by artificial insemination (AI) using 
      cryopreserved boar spermatozoa. Recently, we and other groups reported that high 
      conception rates (70-80%) can be achieved by AI with frozen-thawed boar 
      spermatozoa using a modified temperature program during freezing, or a novel 
      cryopreservation extender to improve sperm quality (including sperm 
      survivability, motility, membrane status and fertilization ability) after 
      thawing, or a novel sperm infusion method, deep intra uterine insemination. 
      However, these techniques have not yet been used for commercial pig production. 
      The variation in sperm freezability among boars or among ejaculations in an 
      identical boar is one of the main reasons for this problem. In our previous 
      study, it was revealed that some components of seminal plasma have a negative 
      effect on the freezability of boar sperm. One of these factors is 
      bacteria-released endotoxin (lipopolysaccharide: LPS). LPS binds to Toll-like 
      receptor-4 (TLR-4) expressed on the sperm surface, resulting in induction of 
      apoptosis. On the other hand, seminal plasma suppresses cryo-capacitation induced 
      by thawing stress. On the basis of these findings, we designed a novel protocol 
      of AI using frozen-thawed boar sperm.
CI  - (c) 2012 Japanese Society of Animal Science.
FAU - Okazaki, Tetsuji
AU  - Okazaki T
AD  - Smaller Livestock and Environment Section, Livestock Research Institute, Oita 
      Prefectural Agriculture, Forestry and Fisheries Research Center, Oita.
FAU - Shimada, Masayuki
AU  - Shimada M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20120703
PL  - Australia
TA  - Anim Sci J
JT  - Animal science journal = Nihon chikusan Gakkaiho
JID - 100956805
SB  - IM
MH  - Animals
MH  - Cryopreservation/methods/*veterinary
MH  - Freezing
MH  - Insemination, Artificial/veterinary
MH  - Male
MH  - Semen/*physiology
MH  - Semen Preservation/methods/*veterinary
MH  - Swine/*physiology
EDAT- 2012/09/05 06:00
MHDA- 2013/10/18 06:00
CRDT- 2012/09/05 06:00
PHST- 2012/09/05 06:00 [entrez]
PHST- 2012/09/05 06:00 [pubmed]
PHST- 2013/10/18 06:00 [medline]
AID - 10.1111/j.1740-0929.2012.01034.x [doi]
PST - ppublish
SO  - Anim Sci J. 2012 Sep;83(9):623-9. doi: 10.1111/j.1740-0929.2012.01034.x. Epub 
      2012 Jul 3.