PMID- 22944006
OWN - NLM
STAT- MEDLINE
DCOM- 20130107
LR  - 20131121
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 906
DP  - 2012 Oct 1
TI  - On-line sample concentration and determination of cationic alkaloids in human 
      plasma by micelle to solvent stacking in capillary zone electrophoresis.
PG  - 41-7
LID - S1570-0232(12)00489-8 [pii]
LID - 10.1016/j.jchromb.2012.08.017 [doi]
AB  - A sensitive method for the determination of three cationic alkaloids (berberine, 
      palmatine and jatrorrhizine) from human plasma samples was developed by micelle 
      to solvent stacking (MSS) in capillary zone electrophoresis (CZE). In MSS, the 
      sample preconcentration mainly relies on the reversal in the effective 
      electrophoretic mobility of the analytes at the boundary zone between the sample 
      and CZE background solution (BGS). Under the optimized conditions, the 
      sensitivity enhancement factors achieved in terms of corrected peak area were in 
      the range from 47 to 53 for the alkaloids. The limits of detection (LODs) (S/N=3) 
      for berberine, palmatine and jatrorrhizine were 0.01, 0.01 and 0.02mug/mL, 
      respectively. The intraday (n=6) and interday repeatabilities (n=12) expressed as 
      the relative standard deviations (RSDs) were less than 6.9% in terms of peak 
      height and less than 7.3% in terms of corrected peak area, respectively. The 
      recoveries of the method for the three alkaloids were in the range of 95.9-101.5% 
      with peak height as the quantitative signal, and 92.6-103.6% with corrected peak 
      area as the quantitative signal, respectively. The MSS-CZE method proved to be 
      suitable for the analysis of the alkaloids in human plasma samples.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Zhang, Shuaihua
AU  - Zhang S
AD  - Key Laboratory of Bioinorganic Chemistry, College of Science, Agricultural 
      University of Hebei, Baoding, Hebei, China.
FAU - Ma, Ruiyang
AU  - Ma R
FAU - Yang, Xiumin
AU  - Yang X
FAU - Wang, Chun
AU  - Wang C
FAU - Wang, Zhi
AU  - Wang Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120821
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Berberine Alkaloids)
RN  - 0 (Bicarbonates)
RN  - 0 (Cations)
RN  - 0 (Micelles)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - 45JP4345C9 (ammonium bicarbonate)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Berberine Alkaloids/*blood
MH  - Bicarbonates/chemistry
MH  - Cations/chemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Humans
MH  - Limit of Detection
MH  - Methanol/chemistry
MH  - Micelles
MH  - Reproducibility of Results
MH  - Sodium Dodecyl Sulfate/chemistry
EDAT- 2012/09/05 06:00
MHDA- 2013/01/08 06:00
CRDT- 2012/09/05 06:00
PHST- 2012/04/04 00:00 [received]
PHST- 2012/07/30 00:00 [revised]
PHST- 2012/08/13 00:00 [accepted]
PHST- 2012/09/05 06:00 [entrez]
PHST- 2012/09/05 06:00 [pubmed]
PHST- 2013/01/08 06:00 [medline]
AID - S1570-0232(12)00489-8 [pii]
AID - 10.1016/j.jchromb.2012.08.017 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Oct 1;906:41-7. doi: 
      10.1016/j.jchromb.2012.08.017. Epub 2012 Aug 21.