PMID- 22944136
OWN - NLM
STAT- MEDLINE
DCOM- 20130425
LR  - 20120904
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 92
IP  - 23
DP  - 2012 Jun 19
TI  - [Preparation of HPV18 E7 peptide plus CpG vaccine and its immunologic effects in 
      vitro].
PG  - 1641-5
AB  - OBJECTIVE: To screen and prepare the vaccine of human papillomavirus (HPV) 18 E7 
      peptide target at human leukocyte antigen (HLA)-A2 plus CpG through SYFPEITHI. 
      METHODS: (1) The SYFPEITHI database was employed for predicting and screening of 
      HPV18 E7 HLA-A2 restricted T cell epitopes.(2) The peripheral blood and tumor 
      tissue sample of HLA-A2 positive and HPV18 positive/negative patients were 
      collected and randomly divided into 7 groups, i.e. E7PA + CpG, E7PB + CpG, E7PC + 
      CpG, E7PD + CpG, CpG, IR-T + CpG and control groups respectively. T cell 
      proliferation was detected by thiazolyl blue tetrazolium bromide (MTT) assay at 
      different timepoints. Lactate dehydrogenase delivery method (LDH) was used to 
      test the cytolytic t lymphocyte (CTL) activity of peripheral blood mononuclear 
      cell (PBMC) in different ratios of effect and target (E:T). And the level of 
      activity T cells was evaluated by interferon gamma (IFN-gamma)-related enzyme-linked 
      immuno-spot assay (ELISPOT). RESULTS: (1) Four peptides named E7PA, E7PB, E7PC 
      and E7PD were obtained separately with high levels of affinity and specificity. 
      (2) During continuous observations after vaccination, the E7PA + CpG group had 
      the most pronounced proliferation rate. When E:T = 100:1, the E7PA + CpG group 
      had more powerful CTL effect than the control group with statistic significance 
      (P < 0.00). E:T was concentration-dependent. Except for IR-T + CpG, all other 
      groups had great difference than control group with statistic significance (P < 
      0.05) but no significant difference between the groups. The levels of IFN-gamma 
      spot-forming T cells were higher in the E7PA + CpG group than the control group 
      with statistic significance (P < 0.01). In terms of specificity, E7PA + CpG in 
      the HPV18 positive group could induce the proliferation of IFN-gamma-secreting T 
      cells. And there was statistical difference with the control group (P < 0.05). 
      CONCLUSION: Screening the HPV18 E7 peptide target at HLA-A2 plus CpG as the 
      candidate targets by SYFPEITHI may active specific immunological cellular 
      responses to HPV18 positive disease.
FAU - Liao, Shu-jie
AU  - Liao SJ
AD  - Department of Gynecologic & Obstetric, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science & Technology, Wuhan 430030, China.
FAU - Zhang, Wei-na
AU  - Zhang WN
FAU - Hu, Xiao-ji
AU  - Hu XJ
FAU - Jiang, Xue-feng
AU  - Jiang XF
FAU - Wang, Chang-yu
AU  - Wang CY
FAU - Lu, Yun-ping
AU  - Lu YP
FAU - Wang, Shi-xuan
AU  - Wang SX
FAU - Ma, Ding
AU  - Ma D
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (E7 protein, Human papillomavirus type 18)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Papillomavirus Vaccines)
RN  - 0 (Peptides)
SB  - IM
MH  - *CpG Islands
MH  - DNA-Binding Proteins/*immunology
MH  - Female
MH  - Humans
MH  - Leukocytes, Mononuclear/immunology
MH  - Oncogene Proteins, Viral/*immunology
MH  - Papillomavirus Vaccines/*immunology
MH  - Peptides/immunology
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Tumor Cells, Cultured
MH  - Uterine Cervical Neoplasms/*immunology/virology
EDAT- 2012/09/05 06:00
MHDA- 2013/04/26 06:00
CRDT- 2012/09/05 06:00
PHST- 2012/09/05 06:00 [entrez]
PHST- 2012/09/05 06:00 [pubmed]
PHST- 2013/04/26 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2012 Jun 19;92(23):1641-5.