PMID- 22946923
OWN - NLM
STAT- MEDLINE
DCOM- 20130122
LR  - 20120919
IS  - 1520-5126 (Electronic)
IS  - 0002-7863 (Linking)
VI  - 134
IP  - 37
DP  - 2012 Sep 19
TI  - Control of protein-binding kinetics on synthetic polymer nanoparticles by tuning 
      flexibility and inducing conformation changes of polymer chains.
PG  - 15209-12
AB  - Although a number of procedures to create synthetic polymer nanoparticles (NPs) 
      with an intrinsic affinity to target biomacromolecules have been published, 
      little has been reported on strategies to control the binding kinetics of target 
      recognition. Here, we report an enzyme-mimic strategy to control 
      binding/dissociation rate constants of NPs, which bind proteins through 
      multipoint interactions, by taking advantage of the temperature-responsive 
      coil-globule phase transition of poly-N-isopropylacrylamide (PNIPAm)-based NPs. 
      PNIPAm NPs with a "flexible" random-coil conformation had a faster binding rate 
      than NPs with a "rigid" globule conformation; however, the dissociation rate 
      constant remained unchanged, resulting in stronger affinity. The dissociation 
      rate of the "flexible" NPs was decelerated by the "induced-fit"-type conformation 
      change of polymers around the coil-globule phase transition temperature, 
      resulting in the formation of the most stable NP-protein complexes. These results 
      provide a guide for designing plastic antibodies with tailor-made binding 
      kinetics and equilibrium constants.
FAU - Hoshino, Yu
AU  - Hoshino Y
AD  - Department of Chemical Engineering, Kyushu University, 744 Motooka, Fukuoka 
      819-0395, Japan. yhoshino@chem-eng.kyushu-u.ac.jp
FAU - Nakamoto, Masahiko
AU  - Nakamoto M
FAU - Miura, Yoshiko
AU  - Miura Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120906
PL  - United States
TA  - J Am Chem Soc
JT  - Journal of the American Chemical Society
JID - 7503056
RN  - 0 (Polymers)
SB  - IM
MH  - Kinetics
MH  - Molecular Conformation
MH  - *Nanoparticles
MH  - Polymers/*chemistry
EDAT- 2012/09/06 06:00
MHDA- 2013/01/23 06:00
CRDT- 2012/09/06 06:00
PHST- 2012/09/06 06:00 [entrez]
PHST- 2012/09/06 06:00 [pubmed]
PHST- 2013/01/23 06:00 [medline]
AID - 10.1021/ja306053s [doi]
PST - ppublish
SO  - J Am Chem Soc. 2012 Sep 19;134(37):15209-12. doi: 10.1021/ja306053s. Epub 2012 
      Sep 6.