PMID- 22947118
OWN - NLM
STAT- MEDLINE
DCOM- 20130328
LR  - 20130201
IS  - 1362-3095 (Electronic)
IS  - 0955-3002 (Linking)
VI  - 89
IP  - 2
DP  - 2013 Feb
TI  - The effect of growth architecture on the induction and decay of bleomycin and 
      X-ray-induced bystander response and genomic instability in lung adenocarcinoma 
      cells and blood lymphocytes.
PG  - 69-78
LID - 10.3109/09553002.2012.726397 [doi]
AB  - PURPOSE: Cancer patients treated with radiomimetic drug bleomycin (BLM) have 
      shown incidence of 7% second malignancy. Studies regarding BLM-induced genomic 
      instability in bystander cells are scarce, and experiments with cells grown on 
      three-dimensional (3D) cultures to mimic the in-vivo condition have never been 
      attempted. MATERIALS AND METHODS: A549 and NCI-H23 (human lung adenocarcinoma) 
      cells were grown as 3D cultures using Cytomatrix(), exposed to BLM or 
      X-radiation and co-cultured with their respective unexposed cells. The DNA damage 
      in direct and bystander cells were assessed by the induction of micronuclei (MN) 
      or phosphorylated serine-15 residue in protein 53 (p53(ser-15)), a reflection of 
      DNA damage, and by up-regulation of protein 21 (p21Waf1). The persistence of DNA 
      damage was measured using MN assay and fluorescence in situ hybridization (FISH) 
      in cancer cells and human peripheral blood lymphocytes (PBL) respectively. 
      RESULTS: BLM or X-irradiation induced DNA damage in both A549 and NCI-H23 cells 
      and their respective bystander cells grown in 2D or 3D cultures. Further 
      persistence of these damages in bystander PBL at delayed times indicated genomic 
      instability in these cells. CONCLUSION: BLM-induced genomic instability in the 
      progeny of bystander cells and their significance in therapy-induced second 
      malignancy may not be eliminated completely.
FAU - Chinnadurai, Mani
AU  - Chinnadurai M
AD  - Department of Human Genetics, College of Biomedical Science Technology and 
      Research , Sri Ramachandra University, Porur, Chennai, India.
FAU - Paul, Solomon F D
AU  - Paul SF
FAU - Venkatachalam, Perumal
AU  - Venkatachalam P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120925
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (Antineoplastic Agents)
RN  - 0 (CDKN1A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (TP53 protein, human)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 11056-06-7 (Bleomycin)
SB  - IM
MH  - Adenocarcinoma/*drug therapy/genetics/pathology/radiotherapy
MH  - Adult
MH  - Antineoplastic Agents/*adverse effects
MH  - Bleomycin/*adverse effects
MH  - Bystander Effect/*drug effects/*radiation effects
MH  - Cell Culture Techniques
MH  - Cell Line, Tumor
MH  - Cyclin-Dependent Kinase Inhibitor p21/genetics
MH  - DNA Damage
MH  - Genomic Instability/*drug effects/*radiation effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*drug therapy/genetics/pathology/radiotherapy
MH  - Lymphocytes/drug effects/radiation effects
MH  - Male
MH  - Micronucleus Tests
MH  - Neoplasms, Second Primary/etiology/genetics
MH  - Translocation, Genetic/drug effects/radiation effects
MH  - Tumor Suppressor Protein p53/chemistry/genetics
EDAT- 2012/09/06 06:00
MHDA- 2013/03/30 06:00
CRDT- 2012/09/06 06:00
PHST- 2012/09/06 06:00 [entrez]
PHST- 2012/09/06 06:00 [pubmed]
PHST- 2013/03/30 06:00 [medline]
AID - 10.3109/09553002.2012.726397 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 2013 Feb;89(2):69-78. doi: 10.3109/09553002.2012.726397. Epub 
      2012 Sep 25.