PMID- 22948796
OWN - NLM
STAT- MEDLINE
DCOM- 20130226
LR  - 20130109
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 158
IP  - 1
DP  - 2013 Jan
TI  - Efficient inhibition of porcine reproductive and respiratory syndrome virus 
      replication by artificial microRNAs targeting the untranslated regions.
PG  - 55-61
LID - 10.1007/s00705-012-1455-z [doi]
AB  - A robust artificial microRNA (amiRNA) strategy against porcine reproductive and 
      respiratory syndrome virus (PRRSV) was developed by targeting the untranslated 
      regions (UTRs). Six candidate amiRNAs targeting the 5' or 3' UTR were used for 
      vector construction, and four effective amiRNAs were selected for further study 
      using a vector transfection/virus infection assay. In cell cultures stably 
      transfected with the four amiRNA vectors, expression of the sequence-specific 
      amiRNAs was confirmed using poly(A)-tailed RT-PCR. After infection with three 
      different PRRSV strains, the viral RNA genome and/or transcript were inhibited by 
      ~90 % (semi-quantitative RT-PCR), and the viral titers were decreased by more 
      than six log CCID(50) (viral titration assay) before day 3 postinfection. The 
      potent anti-PRRSV effects lasted for at least 5 days. Sequence analysis showed 
      that the amiRNA antiviral activities were not compromised by the presence of one 
      or two mismatches in their binding targets. This work constitutes a step towards 
      developing a more effective RNAi strategy against PRRSV.
FAU - Xia, Bing
AU  - Xia B
AD  - College of Veterinary Medicine, Yangzhou University, 48 Wenhui Road, Yangzhou 
      225009, Jiangsu, People's Republic of China.
FAU - Song, Hongqin
AU  - Song H
FAU - Chen, Yang
AU  - Chen Y
FAU - Zhang, Xinyu
AU  - Zhang X
FAU - Xia, Xiaoli
AU  - Xia X
FAU - Sun, Huaichang
AU  - Sun H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120905
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Viral)
RN  - 0 (Untranslated Regions)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Down-Regulation
MH  - MicroRNAs/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/physiology
MH  - RNA Interference
MH  - RNA, Viral/*genetics/metabolism
MH  - Swine
MH  - *Untranslated Regions
MH  - *Virus Replication
EDAT- 2012/09/06 06:00
MHDA- 2013/02/27 06:00
CRDT- 2012/09/06 06:00
PHST- 2012/04/11 00:00 [received]
PHST- 2012/07/14 00:00 [accepted]
PHST- 2012/09/06 06:00 [entrez]
PHST- 2012/09/06 06:00 [pubmed]
PHST- 2013/02/27 06:00 [medline]
AID - 10.1007/s00705-012-1455-z [doi]
PST - ppublish
SO  - Arch Virol. 2013 Jan;158(1):55-61. doi: 10.1007/s00705-012-1455-z. Epub 2012 Sep 
      5.