PMID- 22949349
OWN - NLM
STAT- MEDLINE
DCOM- 20130604
LR  - 20171116
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 114
IP  - 2
DP  - 2013 Feb
TI  - The effect of rotative stress on CAII, FAS, FASL, OSCAR, and TRAP gene expression 
      in osteoclasts.
PG  - 388-97
LID - 10.1002/jcb.24372 [doi]
AB  - This study was designed to explore the effects of rotative stress on carbonic 
      anhydrase II (CAII), TNF receptor superfamily member 6 (FAS), FAS ligand (FASL), 
      osteoclast-associated receptor (OSCAR), and tartrate-resistant acid phosphatase 
      (TRAP) gene expression in osteoclasts. Osteoclasts were induced from RAW264.7 
      cells cultured in medium containing recombinant murine soluble receptor activator 
      of NF-Kbeta ligand (sRANKL). The mRNA and protein expression of CAII, FAS, FASL, 
      OSCAR, and TRAP genes in osteoclasts was detected by RT-PCR and Western blot, 
      respectively, after osteoclasts were loaded at various rotative stress strengths 
      and times. No significant differences in mRNA and protein expression were 
      observed between any of the control groups (P > 0.05). Importantly, rotative 
      stress had a significant effect on the mRNA and protein expression of these genes 
      (P < 0.05). We found a negative relationship between rotative stress strength and 
      prolonged loading time and the expression of FAS/FASL genes in osteoclasts. In 
      addition, there was a positive relationship between rotative stress strength and 
      prolonged loading time and the expression of CAII, OSCAR, or TRAP genes in 
      osteoclasts. Based on these results, rotative stress has a significant effect on 
      CAII, FAS, FASL, OSCAR, and TRAP gene expression in osteoclasts.
CI  - Copyright (c) 2012 Wiley Periodicals, Inc.
FAU - Qing Hong, Zhang
AU  - Qing Hong Z
AD  - Department of Prosthodontics, Stomatology Hospital, College of Medical Sciences, 
      Zhejiang University, No.395 Yan-an Road, Hangzhou 310006, China.
FAU - Meng Tao, Liu
AU  - Meng Tao L
FAU - Yi, Zhou
AU  - Yi Z
FAU - Wei, Liu
AU  - Wei L
FAU - Ju Xiang, Shen
AU  - Ju Xiang S
FAU - Li, Liu
AU  - Li L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Fas Ligand Protein)
RN  - 0 (Fas protein, mouse)
RN  - 0 (Fasl protein, mouse)
RN  - 0 (Isoenzymes)
RN  - 0 (Oscar protein, mouse)
RN  - 0 (RANK Ligand)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Tnfsf11 protein, mouse)
RN  - 0 (fas Receptor)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - EC 3.1.3.2 (Acp5 protein, mouse)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Acid Phosphatase/biosynthesis
MH  - Animals
MH  - Carbonic Anhydrase II/biosynthesis
MH  - Cell Differentiation/*physiology
MH  - Cells, Cultured
MH  - Fas Ligand Protein/biosynthesis
MH  - Gene Expression
MH  - Isoenzymes/biosynthesis
MH  - Mice
MH  - *Osteoclasts/cytology/metabolism
MH  - RANK Ligand/metabolism
MH  - Receptors, Cell Surface/biosynthesis
MH  - *Rotation
MH  - *Stress, Physiological
MH  - Tartrate-Resistant Acid Phosphatase
MH  - fas Receptor/biosynthesis
EDAT- 2012/09/06 06:00
MHDA- 2013/06/05 06:00
CRDT- 2012/09/06 06:00
PHST- 2012/07/02 00:00 [received]
PHST- 2012/08/22 00:00 [accepted]
PHST- 2012/09/06 06:00 [entrez]
PHST- 2012/09/06 06:00 [pubmed]
PHST- 2013/06/05 06:00 [medline]
AID - 10.1002/jcb.24372 [doi]
PST - ppublish
SO  - J Cell Biochem. 2013 Feb;114(2):388-97. doi: 10.1002/jcb.24372.