PMID- 22949597
OWN - NLM
STAT- MEDLINE
DCOM- 20130116
LR  - 20161125
IS  - 1791-7549 (Electronic)
IS  - 0258-851X (Linking)
VI  - 26
IP  - 5
DP  - 2012 Sep-Oct
TI  - Doxorubicin induces early embryo apoptosis by inhibiting poly(ADP ribose) 
      polymerase.
PG  - 827-34
AB  - BACKGROUND: The effect of Doxorubicin, a widely used chemotherapeutic agent, on 
      early mouse embryonic development has not been previously characterized. 
      MATERIALS AND METHODS: Expression of apoptosis-related genes and poly(ADP-ribose) 
      polymerase (PARP) family genes were assessed by real-time reverse transcription 
      polymerase chain reaction (RT-PCR). Apoptosis in mouse blastocysts was tested 
      using the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) 
      assay. Cleaved (c)-PARP was analyzed by western blot. RESULTS: A 20 h exposure to 
      doxorubicin caused rapid cytoplasmic fragmentation, DNA condensation and 
      disruption of the cytoskeleton in mouse embryos. Doxorubicin altered the 
      expression of genes involved in DNA repair and apoptosis and blocked early 
      embryonic development, suggesting that doxorubicin affects DNA synthesis and 
      repair. Furthermore, the effect of doxorubicin on early embryo development was 
      determined by assessing the rates of development to different stages and an 
      apoptosis index. Both assays confirmed that doxorubicin altered embryonic 
      development. In conclusion, doxorubicin blocked pre-implantation development in 
      early mouse embryos by altering apoptosis-related gene expression and 
      inactivating DNA repair by PARP.
FAU - Wang, Qing-Ling
AU  - Wang QL
AD  - Department of Animal Sciences, Chungbuk National University, Chungbuk, Cheongju 
      361-763, South Korea.
FAU - Sun, Shao-Chen
AU  - Sun SC
FAU - Han, Jemesbond
AU  - Han J
FAU - Kwak, Yong-Chul
AU  - Kwak YC
FAU - Kim, Nam-Hyung
AU  - Kim NH
FAU - Cui, Xiang-Shun
AU  - Cui XS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - In Vivo
JT  - In vivo (Athens, Greece)
JID - 8806809
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (Poly(ADP-ribose) Polymerase Inhibitors)
RN  - 80168379AG (Doxorubicin)
RN  - EC 2.4.2.30 (Parp1 protein, mouse)
RN  - EC 2.4.2.30 (Poly (ADP-Ribose) Polymerase-1)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
SB  - IM
MH  - Animals
MH  - Antibiotics, Antineoplastic/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Blastocyst/cytology/*drug effects/enzymology
MH  - DNA Damage
MH  - Doxorubicin/*pharmacology
MH  - Embryo Culture Techniques
MH  - Female
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Poly (ADP-Ribose) Polymerase-1
MH  - *Poly(ADP-ribose) Polymerase Inhibitors
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - Zygote/cytology/drug effects
EDAT- 2012/09/06 06:00
MHDA- 2013/01/17 06:00
CRDT- 2012/09/06 06:00
PHST- 2012/09/06 06:00 [entrez]
PHST- 2012/09/06 06:00 [pubmed]
PHST- 2013/01/17 06:00 [medline]
AID - 26/5/827 [pii]
PST - ppublish
SO  - In Vivo. 2012 Sep-Oct;26(5):827-34.