PMID- 22950075
OWN - NLM
STAT- MEDLINE
DCOM- 20130111
LR  - 20211021
IS  - 2234-3814 (Electronic)
IS  - 2234-3806 (Print)
IS  - 2234-3806 (Linking)
VI  - 32
IP  - 5
DP  - 2012 Sep
TI  - First Korean case of Robinsoniella peoriensis bacteremia in a patient with 
      aspiration pneumonia.
PG  - 370-4
LID - 10.3343/alm.2012.32.5.370 [doi]
AB  - Robinsoniella peoriensis has recently been identified as a Gram-positive, 
      spore-forming, anaerobic rod originally recovered from swine manure storage pits. 
      To date, 6 cases of R. peoriensis infection have been reported, including 2 cases 
      of bacteremia, 1 of abdominal fluid collection, and 3 of wound infection. In the 
      present study, we report a 76-yr-old man with R. peoriensis bacteremia who 
      developed aspiration pneumonia. Gram staining of a purified colony revealed 
      Gram-positive, rod-shaped bacteria. Biochemical identification using API 20 A 
      (bioMerieux, France) indicated presence of Clostridium spp. We performed both 
      500-bp and full-gene sequencing of 16S rRNA of the isolate. The sequence was 
      analyzed with MicroSeq ID 16S rRNA Library v2.0 (Applied Biosystems, USA), 
      GenBank Basic Local Alignment Search Tool (BLAST) 
      (http://www.ncbi.nlm.nih.gov/genbank), and EzTaxon database v2.1 
      (http://www.eztaxon.org). The 500-bp 16S rRNA sequence of the blood culture 
      isolate showed 99.16-99.79% similarity with R. peoriensis and the full-gene 16S 
      rRNA sequence showed 98.87-99.50% similarity with R. peoriensis. The organism was 
      confirmed as R. peoriensis by using all of the mentioned databases except for 
      MicroSeq, which did not include the RNA sequence of this bacterium. This case 
      suggests that identification of R. peoriensis might be challenging in clinical 
      laboratories with no access to molecular methods, as certain commercial 
      identification systems may not identify, or may misidentify, this organism. To 
      the best of our knowledge, this is the first report of the isolation of R. 
      peoriensis in Korea.
FAU - Jeon, Yongbum
AU  - Jeon Y
AD  - Department of Laboratory Medicine, Seoul National University Hospital, Seoul, 
      Korea.
FAU - Kim, Taek Soo
AU  - Kim TS
FAU - Kim, Hong Bin
AU  - Kim HB
FAU - Park, Kyoung Un
AU  - Park KU
FAU - Song, Junghan
AU  - Song J
FAU - Kim, Eui Chong
AU  - Kim EC
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20120813
PL  - Korea (South)
TA  - Ann Lab Med
JT  - Annals of laboratory medicine
JID - 101571172
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Aged
MH  - Bacteremia/*microbiology
MH  - Clostridium/classification/genetics/*isolation & purification
MH  - Databases, Genetic
MH  - Humans
MH  - Male
MH  - Phylogeny
MH  - Pneumonia, Aspiration/*diagnosis/microbiology
MH  - RNA, Ribosomal, 16S/chemistry/genetics
MH  - Republic of Korea
MH  - Sequence Analysis, DNA
PMC - PMC3427827
OTO - NOTNLM
OT  - 16S ribosomal RNA
OT  - Bacteremia
OT  - Robinsoniella peoriensis
COIS- No potential conflicts of interest relevant to this article were reported.
EDAT- 2012/09/06 06:00
MHDA- 2013/01/12 06:00
PMCR- 2012/09/01
CRDT- 2012/09/06 06:00
PHST- 2012/04/09 00:00 [received]
PHST- 2012/04/28 00:00 [revised]
PHST- 2012/07/17 00:00 [accepted]
PHST- 2012/09/06 06:00 [entrez]
PHST- 2012/09/06 06:00 [pubmed]
PHST- 2013/01/12 06:00 [medline]
PHST- 2012/09/01 00:00 [pmc-release]
AID - 10.3343/alm.2012.32.5.370 [doi]
PST - ppublish
SO  - Ann Lab Med. 2012 Sep;32(5):370-4. doi: 10.3343/alm.2012.32.5.370. Epub 2012 Aug 
      13.