PMID- 22950633
OWN - NLM
STAT- MEDLINE
DCOM- 20130419
LR  - 20151119
IS  - 1939-6376 (Electronic)
IS  - 1939-6368 (Linking)
VI  - 58
IP  - 6
DP  - 2012 Dec
TI  - Oxidative stress markers in follicular fluid of women undergoing in vitro 
      fertilization and embryo transfer.
PG  - 301-5
LID - 10.3109/19396368.2012.701367 [doi]
AB  - The aim of the study was to evaluate the levels of lipid and protein peroxidation 
      markers, in the follicular fluids (FF) of 82 patients undergoing in vitro 
      fertilization (IVF). This included, thiobarbituric acid-reactive substances 
      (TBARS), protein carbonyl, and thiol groups. The oxidative stress markers were 
      compared between the pregnancy positive and pregnancy negative patient groups. 
      The two patient groups were compared in terms of their age, body mass index 
      (BMI), cause of infertility, and the plasma hormone levels (AMH, E(2), peak 
      E(2)). Protein carbonyl and thiol groups were estimated using an ELISA assay and 
      with Ellman's reagent (5, 5'-dithiobis-2-nitrobenzoic acid, DTNB), respectively. 
      The mean FF TBARS level of 29 pregnant women was 0.954 +/- 0.420 micromol/l, whereas it 
      was twice as high (1.961 +/- 0.796 micromol/l) in a group of 53 non-pregnant patients 
      (p < 0.0001). In non-pregnant patients, we observed 2-fold elevated levels of 
      protein carbonyl groups when compared to pregnant women (2.969 +/- 0.723 vs. 1.523 
      +/- 0.254; p < 0.0001). The mean age of women and BMI were significantly higher in 
      the pregnancy negative vs. pregnancy positive group. There were no significant 
      differences in protein thiols and in the levels of the hormones tested between 
      patient groups. Our results demonstrate that elevated FF lipid and protein 
      peroxidation level may have a negative impact on IVF outcome. The findings 
      support the idea that increased level of oxidative stress markers in follicular 
      fluid may play an important role in fertility.
FAU - Borowiecka, Marta
AU  - Borowiecka M
AD  - Department of General Biochemistry, University of Lodz, Lodz, Poland.
FAU - Wojsiat, Joanna
AU  - Wojsiat J
FAU - Polac, Ireneusz
AU  - Polac I
FAU - Radwan, Michal
AU  - Radwan M
FAU - Radwan, Pawel
AU  - Radwan P
FAU - Zbikowska, Halina Malgorzata
AU  - Zbikowska HM
LA  - eng
PT  - Journal Article
DEP - 20120905
PL  - England
TA  - Syst Biol Reprod Med
JT  - Systems biology in reproductive medicine
JID - 101464963
RN  - 0 (Biomarkers)
RN  - 0 (Gonadal Steroid Hormones)
SB  - IM
MH  - Adult
MH  - Biomarkers/*analysis
MH  - Case-Control Studies
MH  - *Embryo Transfer
MH  - Female
MH  - *Fertilization in Vitro
MH  - Follicular Fluid/*chemistry
MH  - Gonadal Steroid Hormones/blood
MH  - Humans
MH  - *Oxidative Stress
MH  - Pregnancy
EDAT- 2012/09/07 06:00
MHDA- 2013/04/23 06:00
CRDT- 2012/09/07 06:00
PHST- 2012/09/07 06:00 [entrez]
PHST- 2012/09/07 06:00 [pubmed]
PHST- 2013/04/23 06:00 [medline]
AID - 10.3109/19396368.2012.701367 [doi]
PST - ppublish
SO  - Syst Biol Reprod Med. 2012 Dec;58(6):301-5. doi: 10.3109/19396368.2012.701367. 
      Epub 2012 Sep 5.