PMID- 2295066
OWN - NLM
STAT- MEDLINE
DCOM- 19900213
LR  - 20071114
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 50
IP  - 2
DP  - 1990 Jan 15
TI  - Differential effects of transforming growth factor beta 1 on the growth of poorly 
      and highly metastatic murine melanoma cells.
PG  - 273-7
AB  - We have examined the effects of transforming growth factor beta 1 (TGF-beta 1) on 
      the growth of paired murine melanoma cell clones that differ with respect to 
      their experimental metastatic potential. Neither poorly (clone 16) nor highly 
      (clone M2) metastatic cells were capable of anchorage-independent growth in 0.3% 
      agar/Dulbecco's modified Eagle's medium in the absence of serum. However, both 
      clones were capable of anchorage-independent growth in 0.3% agar/Dulbecco's 
      modified Eagle's medium containing 10% calf serum. Colony formation in the 
      presence of 10% calf serum was enhanced in a dose-dependent manner by TGF-beta 1 
      (half-maximal dose, 0.1 ng/ml) and was 5- to 10-fold greater than colony 
      formation in the presence of 10% calf serum alone. Under anchorage-dependent 
      (monolayer) conditions, neither clone grew in the absence of serum or in medium 
      containing less than 1% calf serum. The monolayer growth of poorly metastatic 
      cells (clone 16) was enhanced in a dose-dependent manner by TGF-beta 1 in medium 
      supplemented with calf serum. Growth was 3.5-fold and 2.3-fold greater than 
      untreated controls after 5 days in submitogenic (0.5%) and mitogenic (10%) 
      concentrations of calf serum, respectively. In contrast, TGF-beta 1 had no effect 
      on the monolayer growth of highly metastatic cells (clone M2) either in 
      submitogenic (0.5%) or mitogenic (10%) concentrations of serum. TGF-beta 1 did 
      not directly stimulate DNA synthesis by either poorly or highly metastatic cells 
      when measured 24 h after TGF-beta 1 treatment. The ability of TGF-beta 1 to 
      stimulate the anchorage-independent growth of metastatic melanoma cells suggests 
      that this potent growth factor may play a role in the growth of these cells in 
      vivo. In addition, the differential sensitivity of poorly and highly metastatic 
      cells to TGF-beta 1 may be relevant to their metastatic potential in vivo. While 
      the mechanism(s) by which TGF-beta 1 stimulates the growth of these cells remains 
      unknown, these differentially metastatic clones of the K-1735 murine melanoma 
      should provide a useful model in which to study the effects of transforming 
      growth factor beta on the metastatic phenotype.
FAU - Mooradian, D L
AU  - Mooradian DL
AD  - Department of Laboratory Medicine and Pathology, University of Minnesota, 
      Minneapolis 55455.
FAU - Purchio, A F
AU  - Purchio AF
FAU - Furcht, L T
AU  - Furcht LT
LA  - eng
GR  - CA21463/CA/NCI NIH HHS/United States
GR  - CA29995/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Culture Media)
RN  - 0 (DNA, Neoplasm)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 76057-06-2 (Transforming Growth Factors)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects
MH  - Culture Media
MH  - DNA, Neoplasm/biosynthesis
MH  - Epidermal Growth Factor/pharmacology
MH  - Melanoma/*pathology
MH  - Mice
MH  - *Neoplasm Metastasis
MH  - Transforming Growth Factors/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1990/01/15 00:00
MHDA- 1990/01/15 00:01
CRDT- 1990/01/15 00:00
PHST- 1990/01/15 00:00 [pubmed]
PHST- 1990/01/15 00:01 [medline]
PHST- 1990/01/15 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1990 Jan 15;50(2):273-7.