PMID- 2295122
OWN - NLM
STAT- MEDLINE
DCOM- 19900222
LR  - 20190510
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 11
IP  - 1
DP  - 1990 Jan
TI  - Cytotoxic and transformation responses of rat tracheal epithelial cells exposed 
      to nitrated polycyclic aromatic hydrocarbons in culture.
PG  - 155-8
AB  - Four nitropolycyclic aromatic hydrocarbons (NPAHs) were investigated for their 
      cytotoxic effects on rat tracheal epithelial (RTE) cells. 6-Nitrochrysene (6-NC), 
      1,6-dinitropyrene (1,6-DNP), 1-nitropyrene (1-NP) and 4-nitropyrene (4-NP) 
      induced dose-dependent decreases in the relative colony-forming efficiency (RCFE) 
      of RTE cells. The compounds could be separated into two groups based on their 
      cytotoxic potencies, a group that displayed high cytotoxic effects (6-NC and 
      1,6-DNP), and a group that displayed low cytotoxic effects (1-NP and 4-NP). The 
      most cytotoxic compound was 6-NC, with an ED50 of 0.13 microM, followed by 
      1,6-DNP, 4-NP and 1-NP with ED50s of 1.25, 8.9 and 9.1 microM, respectively. The 
      most cytotoxic compound (6-NC) and one of the components with low cytotoxicity 
      (1-NP) were assayed for their ability to induce preneoplastic transformation of 
      RTE cells using equally toxic doses of both compounds. The frequencies of 
      transformation induced by 6-NC in cells isolated from control animals or from 
      animals pretreated with 3-methylcholanthrene (3-MC) were 8.4 X 10(-3) and 21.4 X 
      10(-3), respectively. 1-NP did not induce cell transformation. Equally toxic 
      doses of the direct acting carcinogen N-methyl-N'-nitro-N-nitrosoguanidine, used 
      as a positive control, induced transformation frequencies of 8.7 X 10(-3) and 6.4 
      X 10(-3) in cells isolated from control animals or from animals pretreated with 
      3-MC, respectively. These studies show that RTE cells have the metabolic capacity 
      to activate NPAHs to toxic metabolites; thus, the RTE system should be very 
      useful for evaluating the potential toxic effects of this ubiquitous class of 
      airborne pollutants. In addition, the observed differences in cellular toxicity 
      and transformation capabilities of 6-NC and 1-NP were consistent with the results 
      of other studies that demonstrated the greater potency for induction of tumors in 
      animals of 6-NC relative to 1-NP.
FAU - Mitchell, C E
AU  - Mitchell CE
AD  - Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental 
      Research Institute, Albuquerque, NM 87185.
FAU - Thomassen, D G
AU  - Thomassen DG
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (Carcinogens)
RN  - 0 (Chrysenes)
RN  - 0 (Polycyclic Compounds)
RN  - 0 (Pyrenes)
RN  - 12H3O2UGSF (Methylnitronitrosoguanidine)
RN  - 66Q2ZUF83N (1,6-dinitropyrene)
RN  - 82ZK83O33Y (6-nitrochrysene)
RN  - TD1665I8Q4 (1-nitropyrene)
RN  - XOT1408EDI (4-nitropyrene)
SB  - IM
MH  - Animals
MH  - Carcinogens/pharmacology
MH  - Cell Survival/drug effects
MH  - *Cell Transformation, Neoplastic
MH  - Cells, Cultured
MH  - Chrysenes/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Epithelial Cells
MH  - Epithelium/drug effects
MH  - Male
MH  - Methylnitronitrosoguanidine/pharmacology
MH  - Polycyclic Compounds/*pharmacology
MH  - Pyrenes/pharmacology
MH  - Rats
MH  - Rats, Inbred F344
MH  - Trachea/*cytology
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1093/carcin/11.1.155 [doi]
PST - ppublish
SO  - Carcinogenesis. 1990 Jan;11(1):155-8. doi: 10.1093/carcin/11.1.155.