PMID- 22951310
OWN - NLM
STAT- MEDLINE
DCOM- 20130227
LR  - 20121009
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 169
IP  - 1
DP  - 2012 Oct
TI  - Interaction of cellular poly(C)-binding protein 2 with nonstructural protein 1beta 
      is beneficial to Chinese highly pathogenic porcine reproductive and respiratory 
      syndrome virus replication.
PG  - 222-30
LID - S0168-1702(12)00298-5 [pii]
LID - 10.1016/j.virusres.2012.08.002 [doi]
AB  - Non-structural protein1beta (Nsp1beta) of porcine reproductive and respiratory syndrome 
      virus (PRRSV) has been recognized to be involved in suppressing the host innate 
      immune response and mediating viral subgenomic mRNA transcription. In the present 
      study, we have analyzed the interaction of Nsp1beta of Chinese highly pathogenic 
      PRRSV (HP-PRRSV) with cellular poly(C)-binding 2 (PCBP2) by means of the yeast 
      two-hybrid screening in a pulmonary alveolar macrophages (PAMs) cDNA library and 
      co-immunoprecipitation (Co-IP) assay. Our results indicated that the Nsp1beta of the 
      HP-PRRSV is able to bind and interact with cellular PCBP2 strongly in both the 
      infected cells and plasmid transfected cells. Their minimal binding regions were 
      identified to be the residues 85-203 aa (PCPbeta and CTE domains) for the Nsp1beta and 
      the residues 96-168 aa (KH2 domain) for PCBP2, respectively. Next, we used 
      confocal immunofluorescence analysis and discovered that, during PRRSV infection 
      in MARC-145 cells and/or plasmid-transfected cells, the Nsp1beta and PCBP2 mainly 
      colocalized in the cytoplasm and perinuclear pattern. Moreover, the 
      siRNA-mediated silencing of PCBP2 gene in the MARC-145 cells resulted in 
      significant reduction of the virus titer in supernatants as well as viral 
      proteins, while no significant effects on the expression of the type I interferon 
      alpha and interferon beta, suggesting that the interaction of the Nsp1beta with cellular 
      PCBP2 is beneficial to Chinese HP-PRRSV replication in MARC-145 cells.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Wang, Lin
AU  - Wang L
AD  - Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, 
      China Agricultural University, Beijing 100193, People's Republic of China.
FAU - He, Qing
AU  - He Q
FAU - Gao, Yueyi
AU  - Gao Y
FAU - Guo, Xin
AU  - Guo X
FAU - Ge, Xinna
AU  - Ge X
FAU - Zhou, Lei
AU  - Zhou L
FAU - Yang, Hanchun
AU  - Yang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120820
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - *Host-Pathogen Interactions
MH  - Immunoprecipitation
MH  - Macrophages/virology
MH  - Microscopy, Confocal
MH  - Microscopy, Fluorescence
MH  - Porcine respiratory and reproductive syndrome virus
MH  - Protein Binding
MH  - Protein Interaction Mapping
MH  - RNA-Binding Proteins/*metabolism
MH  - Swine
MH  - Two-Hybrid System Techniques
MH  - Viral Nonstructural Proteins/*metabolism
MH  - *Virus Replication
EDAT- 2012/09/07 06:00
MHDA- 2013/02/28 06:00
CRDT- 2012/09/07 06:00
PHST- 2012/05/21 00:00 [received]
PHST- 2012/08/06 00:00 [revised]
PHST- 2012/08/07 00:00 [accepted]
PHST- 2012/09/07 06:00 [entrez]
PHST- 2012/09/07 06:00 [pubmed]
PHST- 2013/02/28 06:00 [medline]
AID - S0168-1702(12)00298-5 [pii]
AID - 10.1016/j.virusres.2012.08.002 [doi]
PST - ppublish
SO  - Virus Res. 2012 Oct;169(1):222-30. doi: 10.1016/j.virusres.2012.08.002. Epub 2012 
      Aug 20.