PMID- 22952281
OWN - NLM
STAT- MEDLINE
DCOM- 20130402
LR  - 20211021
IS  - 1522-1466 (Electronic)
IS  - 1931-857X (Print)
IS  - 1522-1466 (Linking)
VI  - 303
IP  - 11
DP  - 2012 Dec 1
TI  - Regulation of glomerulotubular balance: II: impact of angiotensin II on 
      flow-dependent transport.
PG  - F1507-16
LID - 10.1152/ajprenal.00277.2012 [doi]
AB  - Underlying glomerulotubular balance (GTB) is the impact of axial flow to regulate 
      Na(+) and HCO(3)(-) transport by modulating Na(+)-H(+) exchanger 3 (NHE3) and 
      H-ATPase activity. It is not known whether the cascade of events following a 
      change in flow relies on local angiotensin (ANG II) generation or receptor 
      availability. Mouse tubules were microperfused in vitro at flows of 5 and 20 
      nl/min, and net fluid (J(v)) and HCO(3)(-) (J(HCO3)) absorption and cell height 
      were measured. Na(+) (J(Na)) and Cl(-) (J(Cl)) absorption and changes in 
      microvillous torque were estimated. Raising flow increased Na(+) and HCO(3)(-) 
      reabsorption but did not change either Cl(-) transport or cell volume. Losartan 
      reduced absolute Na(+) and HCO(3)(-) absorption at both low and high flows but 
      did not affect fractional flow-stimulated transport. Compared with controls, in 
      AT(1a) knockout (KO) mouse tubules, 53% of flow-stimulated Na(+) absorption was 
      abolished, but flow-stimulated HCO(3)(-) absorption was retained at similar 
      levels. The remaining flow-stimulated J(HCO3) was eliminated by the H-ATPase 
      inhibitor bafilomycin. Inhibition of the AT(2) receptor by PD123319 increased 
      both J(Na) and J(HCO3) but did not affect flow-mediated fractional changes. NHE3 
      expression at the protein level was reduced in AT(1a) KO mice kidneys. We 
      conclude that 1) although the AT(1a) receptor is necessary for flow to impact 
      NHE3, the effect on H(+)-ATPase is independent of AT(1a); 2) the small 
      flow-mediated changes in cell volume suggest a coordinate flow effect on both 
      luminal and basolateral transporters; and 3) there is no evidence of 
      flow-dependent Cl(-) transport, and thus no evidence for convective paracellular 
      Cl(-) transport in mouse tubules.
FAU - Du, Zhaopeng
AU  - Du Z
AD  - Dept. of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, 
      CT 06520-8026, USA.
FAU - Wan, Laxiang
AU  - Wan L
FAU - Yan, Qingshang
AU  - Yan Q
FAU - Weinbaum, Sheldon
AU  - Weinbaum S
FAU - Weinstein, Alan M
AU  - Weinstein AM
FAU - Wang, Tong
AU  - Wang T
LA  - eng
GR  - R01 DK029857/DK/NIDDK NIH HHS/United States
GR  - R01-DK-29857/DK/NIDDK NIH HHS/United States
GR  - R01-DK62289/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120905
PL  - United States
TA  - Am J Physiol Renal Physiol
JT  - American journal of physiology. Renal physiology
JID - 100901990
RN  - 0 (Angiotensin II Type 1 Receptor Blockers)
RN  - 0 (Bicarbonates)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Macrolides)
RN  - 0 (Receptor, Angiotensin, Type 1)
RN  - 0 (Slc9a3 protein, mouse)
RN  - 0 (Sodium-Hydrogen Exchanger 3)
RN  - 0 (Sodium-Hydrogen Exchangers)
RN  - 11128-99-7 (Angiotensin II)
RN  - 116764-51-3 (bafilomycin A)
RN  - 9NEZ333N27 (Sodium)
RN  - EC 3.6.3.14 (Proton-Translocating ATPases)
RN  - JMS50MPO89 (Losartan)
SB  - IM
MH  - Angiotensin II/*physiology
MH  - Angiotensin II Type 1 Receptor Blockers/pharmacology
MH  - Animals
MH  - Bicarbonates/*metabolism
MH  - Biological Transport/physiology
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Hemostasis/drug effects/*physiology
MH  - In Vitro Techniques
MH  - Kidney Glomerulus/drug effects/*metabolism
MH  - Kidney Tubules/drug effects/*metabolism
MH  - Losartan/pharmacology
MH  - Macrolides/pharmacology
MH  - Mice
MH  - Mice, Knockout
MH  - Models, Animal
MH  - Proton-Translocating ATPases/antagonists & inhibitors/metabolism
MH  - Receptor, Angiotensin, Type 1/deficiency/genetics/metabolism
MH  - Sodium/*metabolism
MH  - Sodium-Hydrogen Exchanger 3
MH  - Sodium-Hydrogen Exchangers/metabolism
PMC - PMC3532483
EDAT- 2012/09/07 06:00
MHDA- 2013/04/03 06:00
PMCR- 2013/12/01
CRDT- 2012/09/07 06:00
PHST- 2012/09/07 06:00 [entrez]
PHST- 2012/09/07 06:00 [pubmed]
PHST- 2013/04/03 06:00 [medline]
PHST- 2013/12/01 00:00 [pmc-release]
AID - ajprenal.00277.2012 [pii]
AID - F-00277-2012 [pii]
AID - 10.1152/ajprenal.00277.2012 [doi]
PST - ppublish
SO  - Am J Physiol Renal Physiol. 2012 Dec 1;303(11):F1507-16. doi: 
      10.1152/ajprenal.00277.2012. Epub 2012 Sep 5.