PMID- 22952738
OWN - NLM
STAT- MEDLINE
DCOM- 20130219
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 8
DP  - 2012
TI  - 17beta-estradiol regulates the sexually dimorphic expression of BDNF and TrkB 
      proteins in the song system of juvenile zebra finches.
PG  - e43687
LID - 10.1371/journal.pone.0043687 [doi]
LID - e43687
AB  - Mature brain derived neurotrophic factor (BDNF) plays critical roles in 
      development of brain structure and function, including neurogenesis, axon growth, 
      cell survival and processes associated with learning. Expression of this peptide 
      is regulated by estradiol (E2). The zebra finch song system is sexually dimorphic 
      - only males sing and the brain regions controlling song are larger and have more 
      cells in males compared to females. Masculinization of this system is partially 
      mediated by E2, and earlier work suggests that BDNF with its high affinity 
      receptor TrkB may also influence this development. The present study evaluated 
      expression of multiple forms of both BDNF and TrkB in the developing song system 
      in juvenile males and females treated with E2 or a vehicle control. Using 
      immunohistochemistry and Western blot analysis, BDNF was detected across the song 
      nuclei of 25-day-old birds. Westerns allowed the pro- and mature forms of BDNF to 
      be individually identified, and proBDNF to be quantified. Several statistically 
      significant effects of sex existed in both the estimated total number of BDNF+ 
      cells and relative concentration of proBDNF, varying across the regions and 
      methodologies. E2 modulated BDNF expression, although the specific nature of the 
      regulation depended on brain region, sex and the technique used. Similarly, TrkB 
      (both truncated and full-length isoforms) was detected by Western blot in the 
      song system of juveniles of both sexes, and expression was regulated by E2. In 
      the context of earlier research on these molecules in the developing song system, 
      this work provides a critical step in describing specific forms of BDNF and TrkB, 
      and how they can be mediated by sex and E2. As individual isoforms of each can 
      have opposing effects on mechanisms, such as cell survival, it will now be 
      important to investigate in depth their specific functions in song system 
      maturation.
FAU - Tang, Yu Ping
AU  - Tang YP
AD  - Neuroscience Program, Departments of Psychology and Zoology, Michigan State 
      University, East Lansing, Michigan, United States of America. tangyupi@msu.edu
FAU - Wade, Juli
AU  - Wade J
LA  - eng
GR  - R01 MH055488/MH/NIMH NIH HHS/United States
GR  - R01 MH096705/MH/NIMH NIH HHS/United States
GR  - R01-MH55488/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120831
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Protein Precursors)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain/cytology/drug effects/metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Count
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Gene Expression Regulation/*drug effects
MH  - Male
MH  - Passeriformes/*metabolism/physiology
MH  - Protein Precursors/metabolism
MH  - Receptor, trkB/*metabolism
MH  - *Sex Characteristics
MH  - Singing/*drug effects
PMC - PMC3432032
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/09/07 06:00
MHDA- 2013/02/21 06:00
PMCR- 2012/08/31
CRDT- 2012/09/07 06:00
PHST- 2012/04/26 00:00 [received]
PHST- 2012/07/23 00:00 [accepted]
PHST- 2012/09/07 06:00 [entrez]
PHST- 2012/09/07 06:00 [pubmed]
PHST- 2013/02/21 06:00 [medline]
PHST- 2012/08/31 00:00 [pmc-release]
AID - PONE-D-12-12110 [pii]
AID - 10.1371/journal.pone.0043687 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(8):e43687. doi: 10.1371/journal.pone.0043687. Epub 2012 Aug 31.