PMID- 22955017
OWN - NLM
STAT- MEDLINE
DCOM- 20130422
LR  - 20121105
IS  - 1879-2448 (Electronic)
IS  - 0043-1354 (Linking)
VI  - 46
IP  - 19
DP  - 2012 Dec 1
TI  - Accumulation of MS2, GA, and Qbeta phages on high density polyethylene (HDPE) and 
      drinking water biofilms under flow/non-flow conditions.
PG  - 6574-84
LID - S0043-1354(12)00535-0 [pii]
LID - 10.1016/j.watres.2012.07.041 [doi]
AB  - Accumulation of enteric viruses on surfaces within a drinking water distribution 
      system was investigated in a reactor using three F-specific RNA bacteriophages 
      (MS2, GA, and Qbeta) as models of human pathogenic viruses. The influence of 
      hydrodynamic versus hydrostatic conditions and the effect of the colonization of 
      HDPE surfaces with two-month-old biofilms were assessed for virus accumulation on 
      surfaces. In order to work under controlled laminar conditions and to study 
      various wall shear stresses at the same time, a new rotating disc reactor was 
      designed. Among the wall shear rates applied in the reactor (450 to 1640 s(-1)) 
      no significant differences were observed concerning both the total number of 
      bacteria, which was found to be around 1.7 x 10(7) cells/cm(2) and the virus 
      concentrations on surfaces were about 3 x 10(4), 5 x 10(5) and 3 x 10(5) eq 
      PFU/cm(2) for MS2, GA and Qbeta phages, respectively. Comparison between static 
      versus dynamic conditions revealed that both Brownian diffusion and convective 
      diffusion were involved in the transport of these soft colloidal particles and an 
      increase reaching about 1 log in virus concentrations measured on surfaces 
      appeared when hydrodynamic conditions where applied. Our results also showed the 
      influence of the colonization by two-month-old drinking water biofilms which led 
      to a change in the level of virus adhesion. The implication of the 
      physico-chemical properties was also underlined since different adhesion profiles 
      were obtained for the three bacteriophages and MS2 phage was found to be the less 
      adherent one whatever the conditions applied.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Pelleieux, Sandra
AU  - Pelleieux S
AD  - Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), UMR 
      7564 CNRS-Universite de Lorraine, Nancy, France.
FAU - Bertrand, Isabelle
AU  - Bertrand I
FAU - Skali-Lami, Salaheddine
AU  - Skali-Lami S
FAU - Mathieu, Laurence
AU  - Mathieu L
FAU - Francius, Gregory
AU  - Francius G
FAU - Gantzer, Christophe
AU  - Gantzer C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120824
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (Drinking Water)
SB  - IM
MH  - Allolevivirus
MH  - *Biofilms
MH  - Drinking Water/*microbiology
MH  - Kinetics
MH  - *RNA Phages
MH  - Surface Properties
MH  - Water Supply
EDAT- 2012/09/08 06:00
MHDA- 2013/04/23 06:00
CRDT- 2012/09/08 06:00
PHST- 2012/02/13 00:00 [received]
PHST- 2012/07/17 00:00 [revised]
PHST- 2012/07/23 00:00 [accepted]
PHST- 2012/09/08 06:00 [entrez]
PHST- 2012/09/08 06:00 [pubmed]
PHST- 2013/04/23 06:00 [medline]
AID - S0043-1354(12)00535-0 [pii]
AID - 10.1016/j.watres.2012.07.041 [doi]
PST - ppublish
SO  - Water Res. 2012 Dec 1;46(19):6574-84. doi: 10.1016/j.watres.2012.07.041. Epub 
      2012 Aug 24.