PMID- 22955832
OWN - NLM
STAT- MEDLINE
DCOM- 20120917
LR  - 20240321
IS  - 1095-9203 (Electronic)
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 337
IP  - 6099
DP  - 2012 Sep 7
TI  - Rad51 is an accessory factor for Dmc1-mediated joint molecule formation during 
      meiosis.
PG  - 1222-5
LID - 10.1126/science.1219379 [doi]
AB  - Meiotic recombination in budding yeast requires two RecA-related proteins, Rad51 
      and Dmc1, both of which form filaments on DNA capable of directing homology 
      search and catalyzing formation of homologous joint molecules (JMs) and strand 
      exchange. With use of a separation-of-function mutant form of Rad51 that retains 
      filament-forming but not JM-forming activity, we show that the JM activity of 
      Rad51 is fully dispensable for meiotic recombination. The corresponding mutation 
      in Dmc1 causes a profound recombination defect, demonstrating Dmc1's JM activity 
      alone is responsible for meiotic recombination. We further provide biochemical 
      evidence that Rad51 acts with Mei5-Sae3 as a Dmc1 accessory factor. Thus, Rad51 
      is a multifunctional protein that catalyzes recombination directly in mitosis and 
      indirectly, via Dmc1, during meiosis.
FAU - Cloud, Veronica
AU  - Cloud V
AD  - Committee on Genetics, University of Chicago, Cummings Life Science Center, 920 
      East 58th Street, Chicago, IL 60637, USA.
FAU - Chan, Yuen-Ling
AU  - Chan YL
FAU - Grubb, Jennifer
AU  - Grubb J
FAU - Budke, Brian
AU  - Budke B
FAU - Bishop, Douglas K
AU  - Bishop DK
LA  - eng
GR  - R01 GM050936/GM/NIGMS NIH HHS/United States
GR  - GM50936/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DMC1 protein, S cerevisiae)
RN  - 0 (DNA, Fungal)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Mei5 protein, S cerevisiae)
RN  - 0 (Mutant Proteins)
RN  - 0 (Recombinases)
RN  - 0 (SAE3 protein, S cerevisiae)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 2.7.7.- (RAD51 protein, S cerevisiae)
RN  - EC 2.7.7.- (Rad51 Recombinase)
SB  - IM
MH  - Cell Cycle Proteins/chemistry/*metabolism
MH  - Chromosomal Proteins, Non-Histone/metabolism
MH  - DNA, Fungal/chemistry/genetics/metabolism
MH  - DNA, Single-Stranded/chemistry/metabolism
MH  - DNA-Binding Proteins/chemistry/*metabolism
MH  - *Meiosis
MH  - Models, Molecular
MH  - Mutant Proteins/chemistry/metabolism
MH  - Nucleic Acid Conformation
MH  - Protein Binding
MH  - Rad51 Recombinase/chemistry/genetics/*metabolism
MH  - Recombinases/metabolism
MH  - *Recombination, Genetic
MH  - Saccharomyces cerevisiae/genetics/*physiology
MH  - Saccharomyces cerevisiae Proteins/chemistry/genetics/*metabolism
PMC - PMC4056682
MID - NIHMS565801
EDAT- 2012/09/08 06:00
MHDA- 2012/09/18 06:00
PMCR- 2014/06/13
CRDT- 2012/09/08 06:00
PHST- 2012/09/08 06:00 [entrez]
PHST- 2012/09/08 06:00 [pubmed]
PHST- 2012/09/18 06:00 [medline]
PHST- 2014/06/13 00:00 [pmc-release]
AID - 337/6099/1222 [pii]
AID - 10.1126/science.1219379 [doi]
PST - ppublish
SO  - Science. 2012 Sep 7;337(6099):1222-5. doi: 10.1126/science.1219379.