PMID- 22955927
OWN - NLM
STAT- MEDLINE
DCOM- 20130102
LR  - 20210202
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 120
IP  - 18
DP  - 2012 Nov 1
TI  - Neutralizing antibodies inhibit HIV-1 transfer from primary dendritic cells to 
      autologous CD4 T lymphocytes.
PG  - 3708-17
LID - 10.1182/blood-2012-03-418913 [doi]
AB  - Dendritic cells (DCs) support only low levels of HIV-1 replication, but have been 
      shown to transfer infectious viral particles highly efficiently to neighboring 
      permissive CD4 T lymphocytes. This mode of cell-to-cell HIV-1 spread may be a 
      predominant mode of infection and dissemination. In the present study, we 
      analyzed the kinetics of fusion, replication, and the ability of HIV-1-specific 
      Abs to inhibit HIV-1 transfer from immature DCs to autologous CD4 T lymphocytes. 
      We found that neutralizing mAbs prevented HIV-1 transfer to CD4 T lymphocytes in 
      trans and in cis, whereas nonneutralizing Abs did not. Neutralizing Abs also 
      significantly decreased HIV-1 replication in DCs, even when added 2 hours after 
      HIV-1 infection. Interestingly, a similar inhibition of HIV-1 replication in DCs 
      was detected with some nonneutralizing Abs and was correlated with DC maturation. 
      We suggest that the binding of HIV-1-specific Abs to FcgammaRs leads to HIV-1 
      inhibition in DCs by triggering DC maturation. This efficient inhibition of HIV-1 
      transfer by Abs highlights the importance of inducing HIV-specific Abs by 
      vaccination directly at the mucosal portal of HIV-1 entry to prevent early 
      dissemination after sexual transmission.
FAU - Su, Bin
AU  - Su B
AD  - Inserm U748, Universite de Strasbourg, Strasbourg, France.
FAU - Xu, Ke
AU  - Xu K
FAU - Lederle, Alexandre
AU  - Lederle A
FAU - Peressin, Maryse
AU  - Peressin M
FAU - Biedma, Marina Elizabeth
AU  - Biedma ME
FAU - Laumond, Geraldine
AU  - Laumond G
FAU - Schmidt, Sylvie
AU  - Schmidt S
FAU - Decoville, Thomas
AU  - Decoville T
FAU - Proust, Alize
AU  - Proust A
FAU - Lambotin, Melanie
AU  - Lambotin M
FAU - Holl, Vincent
AU  - Holl V
FAU - Moog, Christiane
AU  - Moog C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120906
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies, Neutralizing)
SB  - IM
MH  - Antibodies, Neutralizing/*immunology
MH  - CD4-Positive T-Lymphocytes/immunology/*virology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/immunology/*virology
MH  - Flow Cytometry
MH  - HIV Infections/*transmission/*virology
MH  - HIV-1/immunology
MH  - Humans
EDAT- 2012/09/08 06:00
MHDA- 2013/01/03 06:00
CRDT- 2012/09/08 06:00
PHST- 2012/09/08 06:00 [entrez]
PHST- 2012/09/08 06:00 [pubmed]
PHST- 2013/01/03 06:00 [medline]
AID - S0006-4971(20)46205-6 [pii]
AID - 10.1182/blood-2012-03-418913 [doi]
PST - ppublish
SO  - Blood. 2012 Nov 1;120(18):3708-17. doi: 10.1182/blood-2012-03-418913. Epub 2012 
      Sep 6.