PMID- 22956736
OWN - NLM
STAT- MEDLINE
DCOM- 20130125
LR  - 20200226
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 93
IP  - Pt 12
DP  - 2012 Dec
TI  - Incorporation of GP64 into Helicoverpa armigera nucleopolyhedrovirus enhances 
      virus infectivity in vivo and in vitro.
PG  - 2705-2711
LID - 10.1099/vir.0.046458-0 [doi]
AB  - The envelope fusion proteins of baculoviruses, glycoprotein GP64 from group I 
      nucleopolyhedrovirus (NPV) or the F protein from group II NPV and granulovirus, 
      are essential for baculovirus morphogenesis and infectivity. The F protein is 
      considered the ancestral baculovirus envelope fusion protein, while GP64 is a 
      more recent evolutionary introduction into baculoviruses and exhibits higher 
      fusogenic activity than the F protein. Each of the fusion proteins is required by 
      the respective virus to spread infection within larval tissues. A recombinant 
      Helicoverpa armigera NPV (HearNPV) expressing GP64 from Autographa californica 
      multiple nucleopolyhedrovirus, vHaBac-gp64-egfp, was constructed, which still 
      retained the native F protein, and its infectivity was assayed in vivo and in 
      vitro. Analyses by one-step growth curve to determine viral titre and by 
      quantitative PCR to determine viral DNA copy number showed that vHaBac-gp64-egfp 
      was more infectious in vitro than the control, vHaBac-egfp. The polyhedrin gene 
      (polh) was reintroduced into the recombinant viruses and bioassays showed that 
      vHaBac-gp64-polh accelerated the mortality of infected larvae compared with the 
      vHaBac-egfp-polh control, and the LC(50) (median lethal concentration) of 
      vHaBac-gp64-polh was reduced to approximately 20 % of that of vHaBac-egfp-polh. 
      Therefore, incorporation of GP64 into HearNPV budded virions improved virus 
      infectivity both in vivo and in vitro. The construction of this bivalent virus 
      with a more efficient fusion protein could improve the use of baculoviruses in 
      different areas such as gene therapy and biocontrol.
FAU - Shen, Shu
AU  - Shen S
AD  - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of 
      Sciences, Wuhan 430071, PR China.
FAU - Gan, Yinyin
AU  - Gan Y
AD  - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of 
      Sciences, Wuhan 430071, PR China.
FAU - Wang, Manli
AU  - Wang M
AD  - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of 
      Sciences, Wuhan 430071, PR China.
FAU - Hu, Zhihong
AU  - Hu Z
AD  - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of 
      Sciences, Wuhan 430071, PR China.
FAU - Wang, Hualin
AU  - Wang H
AD  - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of 
      Sciences, Wuhan 430071, PR China.
FAU - Deng, Fei
AU  - Deng F
AD  - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of 
      Sciences, Wuhan 430071, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120905
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Occlusion Body Matrix Proteins)
RN  - 0 (Viral Fusion Proteins)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (polyhedrin protein, Nucleopolyhedrovirus)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Genes, Viral
MH  - Lepidoptera/*virology
MH  - Membrane Fusion/genetics/physiology
MH  - Nucleopolyhedroviruses/genetics/*pathogenicity/*physiology
MH  - Occlusion Body Matrix Proteins
MH  - Viral Fusion Proteins/genetics/*physiology
MH  - Viral Structural Proteins/genetics/physiology
MH  - Virulence/genetics/physiology
MH  - Virus Internalization
MH  - Virus Replication/genetics/physiology
EDAT- 2012/09/08 06:00
MHDA- 2013/01/26 06:00
CRDT- 2012/09/08 06:00
PHST- 2012/09/08 06:00 [entrez]
PHST- 2012/09/08 06:00 [pubmed]
PHST- 2013/01/26 06:00 [medline]
AID - 10.1099/vir.0.046458-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2012 Dec;93(Pt 12):2705-2711. doi: 10.1099/vir.0.046458-0. Epub 2012 
      Sep 5.