PMID- 2295699
OWN - NLM
STAT- MEDLINE
DCOM- 19900214
LR  - 20220330
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 85
IP  - 1
DP  - 1990 Jan
TI  - Tumor necrosis factor-alpha inhibits albumin gene expression in a murine model of 
      cachexia.
PG  - 248-55
AB  - The mechanisms responsible for decreased serum albumin levels in patients with 
      cachexia-associated infection, inflammation, and cancer are unknown. Since tumor 
      necrosis factor-alpha (TNF alpha) is elevated in cachexia-associated diseases, 
      and chronic administration of TNF alpha induces cachexia in animal models, we 
      assessed the regulation of albumin gene expression by TNF alpha in vivo. In this 
      animal model of cachexia, Chinese hamster ovary cells transfected with the 
      functional gene for human TNF alpha were inoculated into nude mice (TNF alpha 
      mice). TNF alpha mice became cachectic and manifested decreased serum albumin 
      levels, albumin synthesis, and albumin mRNA levels. However, even before the TNF 
      alpha mice lost weight, their albumin mRNA steady-state levels were decreased 
      approximately 90%, and in situ hybridization revealed a low level of albumin gene 
      expression throughout the hepatic lobule. The mRNA levels of several other genes 
      were unchanged. Hepatic nuclei from TNF alpha mice before the onset of weight 
      loss were markedly less active in transcribing the albumin gene than hepatic 
      nuclei from control mice. Therefore, TNF alpha selectively inhibits the genetic 
      expression of albumin in this model before weight loss.
FAU - Brenner, D A
AU  - Brenner DA
AD  - Department of Medicine, University of California, San Diego 92093.
FAU - Buck, M
AU  - Buck M
FAU - Feitelberg, S P
AU  - Feitelberg SP
FAU - Chojkier, M
AU  - Chojkier M
LA  - eng
GR  - DK07202/DK/NIDDK NIH HHS/United States
GR  - DK38652/DK/NIDDK NIH HHS/United States
GR  - GM41804/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (RNA, Messenger)
RN  - 0 (Serum Albumin)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Cachexia/etiology/*physiopathology
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Disease Models, Animal
MH  - *Gene Expression
MH  - *Genes
MH  - Humans
MH  - Liver/metabolism
MH  - Mice
MH  - Mice, Nude
MH  - Nucleic Acid Hybridization
MH  - RNA, Messenger/genetics
MH  - Serum Albumin/biosynthesis/*genetics
MH  - Transcription, Genetic
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/genetics/*physiology
PMC - PMC296412
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
PMCR- 1990/01/01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
PHST- 1990/01/01 00:00 [pmc-release]
AID - 10.1172/JCI114419 [doi]
PST - ppublish
SO  - J Clin Invest. 1990 Jan;85(1):248-55. doi: 10.1172/JCI114419.