PMID- 22960887
OWN - NLM
STAT- MEDLINE
DCOM- 20130403
LR  - 20211021
IS  - 0971-5916 (Print)
IS  - 0975-9174 (Electronic)
IS  - 0971-5916 (Linking)
VI  - 136
IP  - 2
DP  - 2012 Aug
TI  - Bone marrow cells contribute to tubular epithelium regeneration following acute 
      kidney injury induced by mercuric chloride.
PG  - 211-20
AB  - BACKGROUND & OBJECTIVES: Acute tubular necrosis (ATN) caused by renal ischaemia, 
      renal hypo-perfusion, or nephrotoxic substances is the most common form of acute 
      kidney injury (AKI). There are a few treatment options for this life-threatening 
      disease and the mortality rate exceeds 50 per cent. In critical cases of AKI the 
      only option is renal transplantation. In the present study we evaluated whether 
      bone marrow cells (BMCs) are involved in regeneration of kidney tubules following 
      acute tubular necrosis in the mouse. METHODS: Six to eight week old C57BL6/J and 
      congenic enhanced green fluorescence protein (eGFP) mice were used. The relative 
      contributions of eGFP-expressing BMCs were compared in two different approaches 
      to kidney regeneration in the mercuric chloride (HgCl 2 )-induced mouse model of 
      AKI: induced engraftment and forced engraftment. In vitro differentiation of 
      lineage-depleted (Lin - ) BMCs into renal epithelial cells was also studied. 
      RESULTS: In the forced engraftment approach, BMCs were found to play a role in 
      the regeneration of tubules of renal cortex and outer medulla regions. About 70 
      per cent of donor-derived cells expressed megalin. In vitro culture revealed that 
      Lin - BMCs differentiated into megalin, E-cadherin and cytokeratin-19 (CK-19) 
      expressing renal epithelial cells. INTERPRETATION & CONCLUSIONS: The present 
      results demonstrate that Lin - BMCs may contribute in the regeneration of renal 
      tubular epithelium of HgCl 2 -induced AKI. This study may also suggest a 
      potential role of BMCs in treating AKI.
FAU - Yadav, Neelam
AU  - Yadav N
AD  - Stem Cell Biology Laboratory, National Institute of Immunology, New Delhi, India.
FAU - Rao, Someshwara
AU  - Rao S
FAU - Bhowmik, Dipankar M
AU  - Bhowmik DM
FAU - Mukhopadhyay, Asok
AU  - Mukhopadhyay A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Indian J Med Res
JT  - The Indian journal of medical research
JID - 0374701
RN  - 0 (Low Density Lipoprotein Receptor-Related Protein-2)
RN  - 53GH7MZT1R (Mercuric Chloride)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - *Acute Kidney Injury/chemically induced/surgery
MH  - Animals
MH  - Blood Urea Nitrogen
MH  - *Bone Marrow Transplantation
MH  - Cell Differentiation
MH  - Cell- and Tissue-Based Therapy
MH  - Cells, Cultured
MH  - Creatinine/blood
MH  - Epithelium/*growth & development
MH  - Gene Expression
MH  - Humans
MH  - Kidney Transplantation
MH  - *Kidney Tubules/cytology/growth & development/transplantation
MH  - Low Density Lipoprotein Receptor-Related Protein-2/metabolism
MH  - Mercuric Chloride/toxicity
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Regeneration
PMC - PMC3461732
EDAT- 2012/09/11 06:00
MHDA- 2013/04/04 06:00
PMCR- 2012/08/01
CRDT- 2012/09/11 06:00
PHST- 2012/09/11 06:00 [entrez]
PHST- 2012/09/11 06:00 [pubmed]
PHST- 2013/04/04 06:00 [medline]
PHST- 2012/08/01 00:00 [pmc-release]
AID - IndianJMedRes_2012_136_2_211_100763 [pii]
AID - IJMR-136-211 [pii]
PST - ppublish
SO  - Indian J Med Res. 2012 Aug;136(2):211-20.