PMID- 22961470
OWN - NLM
STAT- MEDLINE
DCOM- 20130326
LR  - 20211021
IS  - 1995-8218 (Electronic)
IS  - 1673-7067 (Print)
IS  - 1995-8218 (Linking)
VI  - 28
IP  - 5
DP  - 2012 Oct
TI  - Neurotrophic effects of 7,8-dihydroxycoumarin in primary cultured rat cortical 
      neurons.
PG  - 493-8
LID - 10.1007/s12264-012-1233-7 [doi]
AB  - OBJECTIVE: Neuronal loss in the central nervous system is central to the 
      occurrence of neurodegenerative diseases. Pharmaceutical companies have devoted 
      much effort to developing new drugs against such diseases, since there are 
      currently no effective drugs for neurodegenerative disease treatment. Promoting 
      the capacity for nerve regeneration is an ideal treatment target. The present 
      study aimed to investigate the neurotrophic effects of 7,8-dihydroxycoumarin 
      (DHC) or daphnetin in primary cultured rat cortical neurons. METHODS: Cortical 
      neurons were identified by microtubule-associated protein 2 (MAP2) 
      immunostaining. Morphological observation was used to measure the average length 
      of neurite outgrowth. MTT and lactate dehydrogenase assays were used to assess 
      neuronal survival. The mRNA expression of MAP2 and brain-derived neurotrophic 
      factor (BDNF) was measured by RT-PCR. RESULTS: MAP2 immunostaining showed that 
      most of the cultured cells were neurons. Compared with the vehicle control group, 
      DHC promoted neurite outgrowth and prolonged neuronal survival time at 
      concentrations ranging from 2 to 8 mumol/L. Expression of both BDNF mRNA and MAP2 
      mRNA was increased in the groups treated with 2, 4 and 8 mumol/L DHC. CONCLUSION: 
      DHC significantly increases neurite outgrowth and promotes neuronal survival in 
      primary cultured rat cortical neurons. The neurotrophic effects of DHC are 
      probably associated with increased BDNF expression.
FAU - Yan, Li
AU  - Yan L
AD  - Department of Pharmacology, School of Medicine, Jinan University, Guangzhou, 
      510632, China.
FAU - Zhou, Xiaowen
AU  - Zhou X
FAU - Zhou, Xing
AU  - Zhou X
FAU - Zhang, Zheng
AU  - Zhang Z
FAU - Luo, Huan-Min
AU  - Luo HM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120518
PL  - Singapore
TA  - Neurosci Bull
JT  - Neuroscience bulletin
JID - 101256850
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Umbelliferones)
RN  - XC84571RD2 (daphnetin)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Survival/drug effects/physiology
MH  - Cerebral Cortex/cytology/*drug effects/physiology
MH  - Dose-Response Relationship, Drug
MH  - Nerve Growth Factors/chemistry/*pharmacology
MH  - Nerve Regeneration/*drug effects/physiology
MH  - Neurons/*drug effects/physiology
MH  - Primary Cell Culture
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Umbelliferones/chemistry/*pharmacology
PMC - PMC5560254
EDAT- 2012/09/11 06:00
MHDA- 2013/03/27 06:00
PMCR- 2013/10/01
CRDT- 2012/09/11 06:00
PHST- 2011/10/31 00:00 [received]
PHST- 2011/12/21 00:00 [accepted]
PHST- 2012/09/11 06:00 [entrez]
PHST- 2012/09/11 06:00 [pubmed]
PHST- 2013/03/27 06:00 [medline]
PHST- 2013/10/01 00:00 [pmc-release]
AID - 1233 [pii]
AID - 10.1007/s12264-012-1233-7 [doi]
PST - ppublish
SO  - Neurosci Bull. 2012 Oct;28(5):493-8. doi: 10.1007/s12264-012-1233-7. Epub 2012 
      May 18.