PMID- 22961721
OWN - NLM
STAT- MEDLINE
DCOM- 20130408
LR  - 20210823
IS  - 1552-4957 (Electronic)
IS  - 1552-4949 (Linking)
VI  - 82
IP  - 6
DP  - 2012 Nov
TI  - Flow cytometry CD45-negative B-NHL: a case report of a diffuse large B-cell 
      lymphoma without extranodal involvement.
PG  - 369-71
LID - 10.1002/cyto.b.21038 [doi]
AB  - BACKGROUND: The loss of CD45, the leukocyte-common antigen, has been described in 
      rare cases of large B-cell lymphoma (LBCL) subtypes with extranodal involvement 
      by immunohistochemical methods. Here we report a case of a patient with LBCL, 
      with no extranodal lesions, which is CD45 negative by flow cytometry (FC) 
      immunophenotyping. METHODS: Immunophenotyping and DNA content analysis was 
      performed by multiparametric FC on lymph node and bone marrow aspirate obtained 
      from a 65 year old male patient. RESULTS: Malignant B-lymphocytes were CD5-, 
      CD10+/++, CD11c-, CD19+, CD20+/++, CD23-, CD34-, CD38-/+, CD45-, CD79b++/+++, 
      BCL2 overexpressed, FMC7++, IgM++/+++, TdT- with Lambda light chain restriction. 
      This pathological cellular population showed near-diploid DNA content, with a 
      high proliferate rate. CONCLUSIONS: To our knowledge, we describe the first case 
      of a CD19+ B-cell non-Hodgkin lymphoma without expression of CD45 detected by FC, 
      and the first case without extranodal involvement presentation. This case is 
      reported not only because it is a rare one but also to raise awareness of FC 
      users of its correct diagnosis.
CI  - Copyright (c) 2012 International Clinical Cytometry Society.
FAU - Palmeira, Carlos
AU  - Palmeira C
AD  - Flow Cytometry Laboratory, Department of Hematology, IPO-Porto, Porto, Portugal. 
      palmeira.ca@gmail.com
FAU - Sousa, Maria Emilia
AU  - Sousa ME
FAU - Godinho, Ines
AU  - Godinho I
FAU - Pires, Ana Marta
AU  - Pires AM
FAU - Mendes, Carlos
AU  - Mendes C
FAU - Martins, Gabriela
AU  - Martins G
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20120907
PL  - United States
TA  - Cytometry B Clin Cytom
JT  - Cytometry. Part B, Clinical cytometry
JID - 101235690
RN  - 0 (Antigens, CD)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin M)
RN  - 0 (MS4A1 protein, human)
RN  - EC 2.7.7.31 (DNA Nucleotidylexotransferase)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
RN  - EC 3.1.3.48 (PTPRC protein, human)
SB  - IM
MH  - Aged
MH  - Antigens, CD/analysis/immunology
MH  - B-Lymphocytes/immunology
MH  - Biopsy
MH  - Bone Marrow/immunology
MH  - Cell Proliferation
MH  - DNA Nucleotidylexotransferase/analysis/immunology
MH  - Flow Cytometry/*methods
MH  - Glycoproteins/analysis
MH  - Humans
MH  - Immunoglobulin M/analysis
MH  - Immunophenotyping/methods
MH  - Leukocyte Common Antigens/analysis/immunology/*metabolism
MH  - Lymph Nodes/immunology
MH  - Lymphoma, Large B-Cell, Diffuse/*diagnosis/immunology/pathology
MH  - Male
EDAT- 2012/09/11 06:00
MHDA- 2013/04/09 06:00
CRDT- 2012/09/11 06:00
PHST- 2011/11/16 00:00 [received]
PHST- 2012/07/26 00:00 [revised]
PHST- 2012/07/27 00:00 [accepted]
PHST- 2012/09/11 06:00 [entrez]
PHST- 2012/09/11 06:00 [pubmed]
PHST- 2013/04/09 06:00 [medline]
AID - 10.1002/cyto.b.21038 [doi]
PST - ppublish
SO  - Cytometry B Clin Cytom. 2012 Nov;82(6):369-71. doi: 10.1002/cyto.b.21038. Epub 
      2012 Sep 7.