PMID- 22961973
OWN - NLM
STAT- MEDLINE
DCOM- 20130916
LR  - 20140512
IS  - 1099-1069 (Electronic)
IS  - 0278-0232 (Linking)
VI  - 31
IP  - 2
DP  - 2013 Jun
TI  - Structural alterations in chronic lymphocytic leukaemia. Cytogenetic and FISH 
      analysis.
PG  - 79-87
LID - 10.1002/hon.2025 [doi]
AB  - In this study, we described cytogenetics and fluorescence in situ hybridization 
      (FISH) analysis performed in chronic lymphocytic leukaemia (CLL) patients with 
      structural alterations. Results were correlated with clinical characteristics. A 
      total of 38 CLL patients: 16 cases with complex and 22 with simple karyotypes 
      were studied. For comparison of clinical parameters, a control group of 78 CLL 
      patients with normal karyotype and without FISH genomic alterations were also 
      evaluated. We found 38 structural abnormalities not previously described in the 
      literature, 28 (74%) of them were translocations. In cases with complex 
      karyotypes, chromosomes 6, 8 and 13 were the most frequently involved in new 
      alterations (nine each), followed by chromosomes 12, 14 and 15 (six each). 
      Chromosome 8p was particularly involved in losses, being 8p21-pter the commonest 
      region of overlap. Cases with simple karyotypes, showed del(6q) as the most 
      frequent alteration (39%). Del(9)(q11) was recurrent in our series. Analysis of 
      clinical parameters showed significant differences in white blood count 
      (p = 0.005) and platelet count (p = 0.015) between patients with structural 
      alterations and the control group. In addition, patients with structural 
      alterations had a significantly shorter time to first treatment (TFT) (29 months) 
      than the control group (69 months) (p = 0.037). Cases with complex karyotypes had 
      a lower proportion of patients in Rai 0 clinical stage (15.4% vs 75%) (p = 0.005) 
      and higher beta2 microglobulin levels (3.3 vs 2.5 microg/mL) (p = 0.037) than those with 
      simple karyotypes. Furthermore, a shorter TFT (13 months) and overall survival 
      (56 months) in the complex karyotypes group compared with controls (69 and 
      144 months, respectively) (p = 0.015 and p = 0.005, respectively) were also 
      found. Our results support the importance of cytogenetic analysis for clinical 
      outcome in CLL and suggest that the diversity of genomic alterations is much 
      greater than previously appreciated.
CI  - Copyright (c) 2012 John Wiley & Sons, Ltd.
FAU - Travella, Ana
AU  - Travella A
AD  - Laboratorio de Genetica, Instituto de Medicina Experimental (IMEX) CONICET- 
      Academia Nacional de Medicina, Buenos Aires, Argentina.
FAU - Ripolles, Lorena
AU  - Ripolles L
FAU - Aventin, Anna
AU  - Aventin A
FAU - Rodriguez, Andrea
AU  - Rodriguez A
FAU - Bezares, Raimundo F
AU  - Bezares RF
FAU - Caballin, Maria R
AU  - Caballin MR
FAU - Slavutsky, Irma
AU  - Slavutsky I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120907
PL  - England
TA  - Hematol Oncol
JT  - Hematological oncology
JID - 8307268
SB  - IM
EIN - Hematol Oncol. 2013 Spe;31(3):168
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cytogenetic Analysis/methods
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/*pathology
MH  - Male
MH  - Middle Aged
EDAT- 2012/09/11 06:00
MHDA- 2013/09/17 06:00
CRDT- 2012/09/11 06:00
PHST- 2012/05/14 00:00 [received]
PHST- 2012/07/27 00:00 [revised]
PHST- 2012/08/02 00:00 [accepted]
PHST- 2012/09/11 06:00 [entrez]
PHST- 2012/09/11 06:00 [pubmed]
PHST- 2013/09/17 06:00 [medline]
AID - 10.1002/hon.2025 [doi]
PST - ppublish
SO  - Hematol Oncol. 2013 Jun;31(2):79-87. doi: 10.1002/hon.2025. Epub 2012 Sep 7.