PMID- 22962228
OWN - NLM
STAT- MEDLINE
DCOM- 20130116
LR  - 20161018
IS  - 1437-4331 (Electronic)
IS  - 1434-6621 (Linking)
VI  - 50
IP  - 9
DP  - 2012 Mar 3
TI  - BCR-ABL fusion protein detection in peripheral blood and bone marrow samples of 
      adult precursor B-cell acute lymphoblastic leukemia patients using the flow 
      cytometric immunobead assay.
PG  - 1657-63
LID - /j/cclm.2012.50.issue-9/cclm-2011-0686/cclm-2011-0686.xml [pii]
LID - 10.1515/cclm-2011-0686 [doi]
AB  - BACKGROUND: The ability to detect the BCR-ABL fusion gene in precursor B-cell 
      acute lymphoblastic leukemia (pB-ALL) is essential for making accurate treatment 
      decisions. METHODS: We used a new flow cytometric immunobead assay for BCR-ABL 
      fusion protein detection in peripheral blood and/or bone marrow samples from 38 
      adult pB-ALL patients and the results were compared with polymerase chain 
      reaction (PCR) detection of BCR-ABL transcript. RESULTS: The fusion protein was 
      detected in peripheral blood and bone marrow samples from seven of the 38 (18%) 
      patients, and results for both the p190 and p210 were confirmed by PCR. One case, 
      which was positive by cytogenetics and fluorescence in situ hybridization (FISH), 
      was negative by PCR but positive by flow cytometry. Another case, which was 
      positive by PCR and negative by flow cytometry, was from a patient on steroid 
      treatment. CONCLUSIONS: The cytometric immunobead assay for BCR-ABL fusion 
      protein detection was found to be suitable for the investigation of pB-ALL 
      patients. This assay is reliable, rapid and simple to use for peripheral blood 
      and bone marrow samples.
FAU - Grigoriou, Eirini E
AU  - Grigoriou EE
AD  - Department of Immunology and Histocompatibility, Evangelismos General Hospital, 
      Athens, Greece. egrigore@otenet.gr
FAU - Psarra, Katerina K
AU  - Psarra KK
FAU - Garofalaki, Maria K
AU  - Garofalaki MK
FAU - Tziotziou, Eirini C
AU  - Tziotziou EC
FAU - Papasteriades, Chryssa A
AU  - Papasteriades CA
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20120303
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow/*metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Fusion Proteins, bcr-abl/*analysis/blood
MH  - Humans
MH  - Immunoassay
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*metabolism/pathology
MH  - ROC Curve
MH  - Young Adult
EDAT- 2012/09/11 06:00
MHDA- 2013/01/17 06:00
CRDT- 2012/09/11 06:00
PHST- 2011/09/21 00:00 [received]
PHST- 2012/02/06 00:00 [accepted]
PHST- 2012/09/11 06:00 [entrez]
PHST- 2012/09/11 06:00 [pubmed]
PHST- 2013/01/17 06:00 [medline]
AID - /j/cclm.2012.50.issue-9/cclm-2011-0686/cclm-2011-0686.xml [pii]
AID - 10.1515/cclm-2011-0686 [doi]
PST - epublish
SO  - Clin Chem Lab Med. 2012 Mar 3;50(9):1657-63. doi: 10.1515/cclm-2011-0686.