PMID- 22963628
OWN - NLM
STAT- MEDLINE
DCOM- 20130923
LR  - 20190823
IS  - 1875-533X (Electronic)
IS  - 0929-8673 (Linking)
VI  - 19
IP  - 29
DP  - 2012
TI  - Dendrimer-modified solid supports: nanostructured materials with potential drug 
      allergy diagnostic applications.
PG  - 4942-54
AB  - Complex functional materials consisting of bioactive molecules immobilized on 
      solid supports present potential applications in biosensoring. Advances in the 
      fabrication of these surface materials are of growing interest for antibody-based 
      diagnosis. This work exploits dendrimers as versatile nanostructures for 
      templating sensor surfaces and the critical role of the immobilization protocol 
      in the solid supports cellulose and zeolites, of organic and inorganic 
      composition respectively. The fabrication and characterization, including the 
      degree of functionalization and reproducibility, of different nanostructured 
      materials are described. To validate the approach, the fabricated supports were 
      further used as a solid phase for developing a radioimmunoassay to detect 
      immunoglobulin E (IgE) specific to penicillin, the antibody involved in immediate 
      allergy responses to this drug. The dendrimer-modified supports provide assays 
      with significantly enhanced sensitivity, as well as increase the availability of 
      biomolecules for specific interaction and minimize nonspecific adsorptions 
      through appropriate functionalization protocols in each case. The manufacturing 
      methodology involved the use of a long, flexible hydrophilic spacer in the 
      cellulose materials, and a higher surface density of the immobilized dendrimers 
      in the zeolite crystals. The ability of hybrid zeolite materials in such 
      biosensing applications was evaluated for the first time. The assays were 
      validated in human serum samples from patients allergic to penicillin and from 
      non-allergic controls. The specificity and improved sensitivity of the dendrimer- 
      modified supports make these strategies versatile for different bioactive 
      molecules and could have significant implications for the quantification of a 
      wide range of specific IgE antibodies and other biomolecules of diagnostic 
      interest.
FAU - Ruiz-Sanchez, A J
AU  - Ruiz-Sanchez AJ
AD  - Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, Parque Tecnologico 
      de Andalucia, 29590 Malaga, Spain.
FAU - Montanez, M I
AU  - Montanez MI
FAU - Mayorga, C
AU  - Mayorga C
FAU - Torres, M J
AU  - Torres MJ
FAU - Kehr, N S
AU  - Kehr NS
FAU - Vida, Y
AU  - Vida Y
FAU - Collado, D
AU  - Collado D
FAU - Najera, F
AU  - Najera F
FAU - De Cola, L
AU  - De Cola L
FAU - Perez-Inestrosa, E
AU  - Perez-Inestrosa E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United Arab Emirates
TA  - Curr Med Chem
JT  - Current medicinal chemistry
JID - 9440157
RN  - 0 (Dendrimers)
RN  - 0 (Penicillins)
RN  - 1318-02-1 (Zeolites)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9004-34-6 (Cellulose)
SB  - IM
MH  - Antibody Specificity
MH  - Cellulose/chemistry
MH  - Dendrimers/*chemistry
MH  - Drug Hypersensitivity/*diagnosis
MH  - Humans
MH  - Hypersensitivity, Immediate/chemically induced/*diagnosis
MH  - Immunoglobulin E/immunology
MH  - Nanostructures/*chemistry
MH  - Penicillins/adverse effects/immunology
MH  - Radioallergosorbent Test/*instrumentation/*methods
MH  - Surface Properties
MH  - Zeolites/*chemistry
EDAT- 2012/09/12 06:00
MHDA- 2013/09/24 06:00
CRDT- 2012/09/12 06:00
PHST- 2012/01/30 00:00 [received]
PHST- 2012/05/28 00:00 [revised]
PHST- 2012/08/02 00:00 [accepted]
PHST- 2012/09/12 06:00 [entrez]
PHST- 2012/09/12 06:00 [pubmed]
PHST- 2013/09/24 06:00 [medline]
AID - CMC-EPUB-20120903-10 [pii]
AID - 10.2174/0929867311209024942 [doi]
PST - ppublish
SO  - Curr Med Chem. 2012;19(29):4942-54. doi: 10.2174/0929867311209024942.