PMID- 22966359
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 1
IP  - 4
DP  - 2010 Jul
TI  - Role and mechanism of hypoxia-inducible factor-1 in cell growth and apoptosis of 
      breast cancer cell line MDA-MB-231.
PG  - 657-662
AB  - The role of hypoxia-inducible factor-1 (HIF-1) in tumor development and 
      progression is well-established but its effect on tumor growth remains 
      controversial. The present study investigated the effect of HIF-1 on tumor growth 
      using the estrogen receptor-negative breast cancer cell line, MDA-MB-231. Using 
      Western blotting, we detected a higher level of HIF-1alpha protein in MDA-MB-231 
      cells than in any other breast cancer cell lines analyzed, and this was 
      accompanied by a more rapid growth pattern. Interruption of HIF-1alpha expression 
      using small interference RNA (siRNA) significantly suppressed cell growth and 
      increased apoptosis, but the cell cycle was not affected. Activated fragments 
      with increased caspase 3 activity and a mobility shift of B cell lymphoma (Bcl-2) 
      were also detected in cells treated with HIF-1alpha siRNA. HIF-1 allows breast cancer 
      cells to grow under long-term serum deprivation by inactivation of the caspase 
      cascade and thus inhibition of apoptosis. Blocking HIF-1alpha protein resulted in 
      loss of Bcl-2 function, which may contribute to the activation of the caspase 
      cascade.
FAU - Shi, Yonghong
AU  - Shi Y
AD  - Department of Pathology, Affiliated Hospital, Inner Mongolia Medical College, 
      P.R. China.
FAU - Chang, Miaomiao
AU  - Chang M
FAU - Wang, Fang
AU  - Wang F
FAU - Ouyang, Xiaohui
AU  - Ouyang X
FAU - Jia, Yongfeng
AU  - Jia Y
FAU - DU, Hua
AU  - DU H
LA  - eng
PT  - Journal Article
DEP - 20100701
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC3436333
EDAT- 2010/07/01 00:00
MHDA- 2010/07/01 00:01
PMCR- 2010/07/01
CRDT- 2012/09/12 06:00
PHST- 2010/01/26 00:00 [received]
PHST- 2010/04/21 00:00 [accepted]
PHST- 2012/09/12 06:00 [entrez]
PHST- 2010/07/01 00:00 [pubmed]
PHST- 2010/07/01 00:01 [medline]
PHST- 2010/07/01 00:00 [pmc-release]
AID - ol-01-04-0657 [pii]
AID - 10.3892/ol_00000115 [doi]
PST - ppublish
SO  - Oncol Lett. 2010 Jul;1(4):657-662. doi: 10.3892/ol_00000115. Epub 2010 Jul 1.