PMID- 22966385
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 1
IP  - 5
DP  - 2010 Sep
TI  - Genetic diagnosis of patients with esophageal cancer using FISH.
PG  - 809-813
AB  - This study aimed to the clarify the diagnostic efficacy of fluorescence in situ 
      hybridization (FISH) in Kazakh patients with esophageal cancer (EC). FISH was 
      compared with the pathological examination of biopsy specimens with DNA probes. 
      We enrolled 20 patients, of which 15 were males and 5 females, with an average 
      age of 58.3 years, who had abnormal esophaguses on barium radiological digital 
      imaging. Touch preparations were performed on biopsy specimens from all of the 
      patients and were examined using FISH for chromosomal abnormalities. We compared 
      the FISH results with the pathology slides stained with hematoxylin and eosin. 
      Classification, according to pathology, identified 2 cases of class II, 3 cases 
      of IIIa, 1 case of IIIb, 2 cases of IV, 12 cases of class V and no cases of class 
      I. The cases classified as class IIIb or higher were considered to be positive 
      for cancer. Using histopathology, 10 cases were diagnosed with squamous cell 
      carcinoma and 5 were diagnosed as adenocarcinoma, with one case being 
      false-negative. Thus, the sensitivity of the pathological examination was 93% and 
      the specificity was 100%. Using FISH, 16 cases showed aberrant copy numbers in 
      either chromosome 3 or 17. By comparison, pathology did not reveal any 
      false-positive or false-negative cases with a sensitivity and specificity of 
      100%. The centromeres of chromosome 3 copy numbers was significantly higher 
      (p=0.035) than the centromeres of chromosome 17. Our study compared FISH to 
      diagnose aneusomic esophageal cancer cells with the pathology of biopsied tissue. 
      Our findings suggest that FISH is a useful and objective assay for the detection 
      of malignant cells of esophageal cancer. In our study, the centromeres of 
      chromosome 3 was the more sensitive probe for the diagnosis of esophageal cancer 
      in Kazakh patients.
FAU - Awut, Idiris
AU  - Awut I
AD  - Thoracic Surgery, Medical Research Center, Xinjiang 830054, P.R. China.
FAU - Niyaz, Madiniyet
AU  - Niyaz M
FAU - Huizhong, Xie
AU  - Huizhong X
FAU - Biekemitoufu, Hadeti
AU  - Biekemitoufu H
FAU - Yan, Zhang Hong
AU  - Yan ZH
FAU - Zhu, Zhang
AU  - Zhu Z
FAU - Sheyhedin, Ilyar
AU  - Sheyhedin I
FAU - Changmin, Zhang
AU  - Changmin Z
FAU - Wei, Zhangli
AU  - Wei Z
FAU - Hao, Wen
AU  - Hao W
LA  - eng
PT  - Journal Article
DEP - 20100901
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC3436409
EDAT- 2010/09/01 00:00
MHDA- 2010/09/01 00:01
PMCR- 2010/09/01
CRDT- 2012/09/12 06:00
PHST- 2010/04/29 00:00 [received]
PHST- 2010/06/21 00:00 [accepted]
PHST- 2012/09/12 06:00 [entrez]
PHST- 2010/09/01 00:00 [pubmed]
PHST- 2010/09/01 00:01 [medline]
PHST- 2010/09/01 00:00 [pmc-release]
AID - ol-01-05-0809 [pii]
AID - 10.3892/ol_00000142 [doi]
PST - ppublish
SO  - Oncol Lett. 2010 Sep;1(5):809-813. doi: 10.3892/ol_00000142. Epub 2010 Sep 1.