PMID- 22967311
OWN - NLM
STAT- MEDLINE
DCOM- 20130317
LR  - 20211021
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 9
DP  - 2012 Sep 11
TI  - Viral metagenomic analysis of bushpigs (Potamochoerus larvatus) in Uganda 
      identifies novel variants of Porcine parvovirus 4 and Torque teno sus virus 1 and 
      2.
PG  - 192
LID - 10.1186/1743-422X-9-192 [doi]
AB  - BACKGROUND: As a result of rapidly growing human populations, intensification of 
      livestock production and increasing exploitation of wildlife habitats for animal 
      agriculture, the interface between wildlife, livestock and humans is expanding, 
      with potential impacts on both domestic animal and human health. Wild animals 
      serve as reservoirs for many viruses, which may occasionally result in novel 
      infections of domestic animals and/or the human population. Given this 
      background, we used metagenomics to investigate the presence of viral pathogens 
      in sera collected from bushpigs (Potamochoerus larvatus), a nocturnal species of 
      wild Suid known to move between national parks and farmland, in Uganda. RESULTS: 
      Application of 454 pyrosequencing demonstrated the presence of Torque teno sus 
      virus (TTSuV), porcine parvovirus 4 (PPV4), porcine endogenous retrovirus (PERV), 
      a GB Hepatitis C-like virus, and a Sclerotinia hypovirulence-associated-like 
      virus in sera from the bushpigs. PCR assays for each specific virus combined with 
      Sanger sequencing revealed two TTSuV-1 variants, one TTSuV-2 variant as well as 
      PPV4 in the serum samples and thereby confirming the findings from the 454 
      sequencing. CONCLUSIONS: Using a viral metagenomic approach we have made an 
      initial analysis of viruses present in bushpig sera and demonstrated for the 
      first time the presence of PPV4 in a wild African Suid. In addition we identified 
      novel variants of TTSuV-1 and 2 in bushpigs.
FAU - Blomstrom, Anne-Lie
AU  - Blomstrom AL
AD  - Department of Biomedical Sciences and Veterinary Public Health, Swedish 
      University of Agricultural Sciences, Uppsala, Sweden. anne-lie.blomstrom@slu.se
FAU - Stahl, Karl
AU  - Stahl K
FAU - Masembe, Charles
AU  - Masembe C
FAU - Okoth, Edward
AU  - Okoth E
FAU - Okurut, Ademun Rose
AU  - Okurut AR
FAU - Atmnedi, Patrick
AU  - Atmnedi P
FAU - Kemp, Stephen
AU  - Kemp S
FAU - Bishop, Richard
AU  - Bishop R
FAU - Belak, Sandor
AU  - Belak S
FAU - Berg, Mikael
AU  - Berg M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120911
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
SB  - IM
MH  - Animals
MH  - DNA Virus Infections/epidemiology/veterinary/virology
MH  - Genome, Viral
MH  - Genomics/*methods
MH  - Parvovirus, Porcine/*classification/*genetics
MH  - Phylogeny
MH  - *Swine
MH  - Swine Diseases/epidemiology/virology
MH  - Torque teno virus/*classification/*genetics
MH  - Uganda/epidemiology
PMC - PMC3478234
EDAT- 2012/09/13 06:00
MHDA- 2013/03/19 06:00
PMCR- 2012/09/11
CRDT- 2012/09/13 06:00
PHST- 2012/03/19 00:00 [received]
PHST- 2012/09/05 00:00 [accepted]
PHST- 2012/09/13 06:00 [entrez]
PHST- 2012/09/13 06:00 [pubmed]
PHST- 2013/03/19 06:00 [medline]
PHST- 2012/09/11 00:00 [pmc-release]
AID - 1743-422X-9-192 [pii]
AID - 10.1186/1743-422X-9-192 [doi]
PST - epublish
SO  - Virol J. 2012 Sep 11;9:192. doi: 10.1186/1743-422X-9-192.