PMID- 22967313
OWN - NLM
STAT- MEDLINE
DCOM- 20130405
LR  - 20221207
IS  - 1751-7915 (Electronic)
IS  - 1751-7915 (Print)
IS  - 1751-7915 (Linking)
VI  - 5
IP  - 6
DP  - 2012 Nov
TI  - Microbial community structure and dynamics in anaerobic fluidized-bed and 
      granular sludge-bed reactors: influence of operational temperature and reactor 
      configuration.
PG  - 738-52
LID - 10.1111/j.1751-7915.2012.00364.x [doi]
AB  - Methanogenic community structure and dynamics were investigated in two different, 
      replicated anaerobic wastewater treatment reactor configurations [inverted 
      fluidized bed (IFB) and expanded granular sludge bed (EGSB)] treating synthetic 
      dairy wastewater, during operating temperature transitions from 37 degrees C to 25 degrees C, and 
      from 25 degrees C to 15 degrees C, over a 430-day trial. Non-metric multidimensional scaling 
      (NMS) and moving-window analyses, based on quantitative real-time PCR data, along 
      with denaturing gradient gel electrophoresis (DGGE) profiling, demonstrated that 
      the methanogenic communities developed in a different manner in these reactor 
      configurations. A comparable level of performance was recorded for both systems 
      at 37 degrees C and 25 degrees C, but a more dynamic and diverse microbial community in the IFB 
      reactors supported better stability and adaptative capacity towards low 
      temperature operation. The emergence and maintenance of particular bacterial 
      genotypes (phylum Firmicutes and Bacteroidetes) was associated with efficient 
      protein hydrolysis in the IFB, while protein hydrolysis was inefficient in the 
      EGSB. A significant community shift from a Methanobacteriales and 
      Methanosaetaceae towards a Methanomicrobiales-predominated community was 
      demonstrated during operation at 15 degrees C in both reactor configurations.
CI  - (c) 2012 The Authors. Microbial Biotechnology (c) 2012 Society for Applied 
      Microbiology and Blackwell Publishing Ltd.
FAU - Bialek, Katarzyna
AU  - Bialek K
AD  - Microbial Ecology Laboratory, Microbiology, School of Natural Sciences and Ryan 
      Institute, National University of Ireland, Galway, Ireland.
FAU - Kumar, Amit
AU  - Kumar A
FAU - Mahony, Therese
AU  - Mahony T
FAU - Lens, Piet N L
AU  - Lens PN
FAU - O'Flaherty, Vincent
AU  - O'Flaherty V
LA  - eng
SI  - GENBANK/JF927800
SI  - GENBANK/JF927801
SI  - GENBANK/JF927802
SI  - GENBANK/JF927803
SI  - GENBANK/JF927804
SI  - GENBANK/JF927805
SI  - GENBANK/JF927806
SI  - GENBANK/JF927807
SI  - GENBANK/JF927808
SI  - GENBANK/JF927809
SI  - GENBANK/JF927810
SI  - GENBANK/JF927811
SI  - GENBANK/JF927812
SI  - GENBANK/JF927813
SI  - GENBANK/JF927814
SI  - GENBANK/JF927815
SI  - GENBANK/JF927816
SI  - GENBANK/JF927817
SI  - GENBANK/JF927818
SI  - GENBANK/JF927819
SI  - GENBANK/JF927820
SI  - GENBANK/JF927821
SI  - GENBANK/JF927822
SI  - GENBANK/JF927823
SI  - GENBANK/JF927824
SI  - GENBANK/JF927825
SI  - GENBANK/JF927826
SI  - GENBANK/JF927827
SI  - GENBANK/JF927828
SI  - GENBANK/JF927829
SI  - GENBANK/JF952003
SI  - GENBANK/JF952004
SI  - GENBANK/JF952005
SI  - GENBANK/JF952006
SI  - GENBANK/JF952007
SI  - GENBANK/JF952008
SI  - GENBANK/JF952009
SI  - GENBANK/JF952010
SI  - GENBANK/JF952011
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120911
PL  - United States
TA  - Microb Biotechnol
JT  - Microbial biotechnology
JID - 101316335
RN  - 0 (DNA, Bacterial)
RN  - 0 (Sewage)
RN  - 0 (Waste Water)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Bacteria/*classification/*genetics
MH  - *Biota
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - Dairy Products
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Food Industry
MH  - Methane/metabolism
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
MH  - Temperature
MH  - Wastewater/*microbiology
MH  - Water Purification
PMC - PMC3815895
EDAT- 2012/09/13 06:00
MHDA- 2013/04/06 06:00
PMCR- 2012/11/01
CRDT- 2012/09/13 06:00
PHST- 2012/04/12 00:00 [received]
PHST- 2012/07/06 00:00 [revised]
PHST- 2012/08/06 00:00 [accepted]
PHST- 2012/09/13 06:00 [entrez]
PHST- 2012/09/13 06:00 [pubmed]
PHST- 2013/04/06 06:00 [medline]
PHST- 2012/11/01 00:00 [pmc-release]
AID - 10.1111/j.1751-7915.2012.00364.x [doi]
PST - ppublish
SO  - Microb Biotechnol. 2012 Nov;5(6):738-52. doi: 10.1111/j.1751-7915.2012.00364.x. 
      Epub 2012 Sep 11.