PMID- 22967488
OWN - NLM
STAT- MEDLINE
DCOM- 20130222
LR  - 20161125
IS  - 1872-678X (Electronic)
IS  - 0165-0270 (Linking)
VI  - 210
IP  - 2
DP  - 2012 Sep 30
TI  - Evaluation of transcranial Doppler flow velocity changes in intracerebral 
      hemorrhage rats using ultrasonography.
PG  - 272-80
LID - S0165-0270(12)00288-9 [pii]
LID - 10.1016/j.jneumeth.2012.07.024 [doi]
AB  - This study investigated the blood flow velocity changes in seven major arteries 
      in rat brain before and after intracerebral hemorrhage (ICH) using high frequency 
      transcranial Doppler (TCD) ultrasonography (13-4 MHz). Eighteen adult Sprague 
      Dawley rats received either the collagenase-injection surgery (ICH, n=12) or the 
      saline-injection surgery (control, n=6) after baseline TCD flow velocity values 
      were recorded. The TCD flow velocity changes were measured at 0.5h after the 
      surgery and daily for the following 8 days in seven major cerebral arteries, 
      including bilateral internal carotid arteries, bilateral middle cerebral arteries 
      (MCAs), bilateral posterior cerebral arteries (PCAs), and basilar artery. The 
      results showed a significant decrease of TCD flow velocity in the right MCA at 
      0.5 h and 24 h after the collagenase-injection surgery, and in the right PCA at 
      0.5 h in the ICH group rats. The TCD flow velocities in these two arteries 
      gradually increased and then returned to the baseline values in the following 
      days. The control group rats did not show significant changes in TCD flow 
      velocity in all monitored arteries. This study demonstrates the feasibility and 
      reliability of monitoring TCD flow velocity in cerebral arteries using 
      ultrasonography technique in a rat ICH model. The results of this study extend 
      our knowledge in the cerebrovascular changes during intracranial hemorrhage and 
      suggest a possibility of clinical application of TCD ultrasonography in studying 
      the dynamic cerebral circulation after strokes. Moreover, this method could be 
      extensively applied in further studies using potential neuroprotective treatments 
      that affect the cerebral dynamics in the intracerebral hemorrhage.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Ke, Zheng
AU  - Ke Z
AD  - Interdisciplinary Division of Biomedical Engineering, The Hong Kong Polytechnic 
      University, Hong Kong Special Administrative Region.
FAU - Ying, Michael
AU  - Ying M
FAU - Li, Le
AU  - Li L
FAU - Zhang, Shaojie
AU  - Zhang S
FAU - Tong, Kai-yu
AU  - Tong KY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120809
PL  - Netherlands
TA  - J Neurosci Methods
JT  - Journal of neuroscience methods
JID - 7905558
RN  - 0 (Enzyme Inhibitors)
RN  - EC 3.4.24.- (Collagenases)
SB  - IM
MH  - Animals
MH  - Blood Flow Velocity/physiology
MH  - Brain/drug effects/pathology
MH  - Cerebral Hemorrhage/*diagnostic imaging/drug therapy/physiopathology
MH  - Cerebrovascular Circulation/drug effects/*physiology
MH  - Collagenases/therapeutic use
MH  - Disease Models, Animal
MH  - Enzyme Inhibitors/therapeutic use
MH  - Heart Rate/drug effects
MH  - Male
MH  - Middle Cerebral Artery/diagnostic imaging/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reproducibility of Results
MH  - Ultrasonography, Doppler, Transcranial/*methods
EDAT- 2012/09/13 06:00
MHDA- 2013/02/23 06:00
CRDT- 2012/09/13 06:00
PHST- 2012/03/31 00:00 [received]
PHST- 2012/07/12 00:00 [revised]
PHST- 2012/07/30 00:00 [accepted]
PHST- 2012/09/13 06:00 [entrez]
PHST- 2012/09/13 06:00 [pubmed]
PHST- 2013/02/23 06:00 [medline]
AID - S0165-0270(12)00288-9 [pii]
AID - 10.1016/j.jneumeth.2012.07.024 [doi]
PST - ppublish
SO  - J Neurosci Methods. 2012 Sep 30;210(2):272-80. doi: 
      10.1016/j.jneumeth.2012.07.024. Epub 2012 Aug 9.