PMID- 22967640
OWN - NLM
STAT- MEDLINE
DCOM- 20130212
LR  - 20131121
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 99
DP  - 2012 Sep 15
TI  - Selective extraction and determination of catecholamines in urine samples by 
      using a dopamine magnetic molecularly imprinted polymer and capillary 
      electrophoresis.
PG  - 897-903
LID - 10.1016/j.talanta.2012.07.053 [doi]
AB  - A rapid and selective method for the extraction and determination of 
      catecholamines (CLs) from urine samples has been successfully developed using a 
      magnetic molecularly imprinted polymer (MMIP) as a sorbent material. The MMIP has 
      been prepared using dopamine hydrochloride (DA) as template molecule, methacrylic 
      acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EDMA) as 
      cross-linking agent and Fe(3)O(4) magnetite as magnetic component. The extraction 
      was carried out by stirring urine samples with the magnetic polymer. When the 
      extraction was completed, the MMIP, together with the captured analytes, was 
      easily separated from the sample matrix by an adscititious magnet. The analytes 
      desorbed from the MMIP were determined by capillary electrophoresis (CE). It was 
      shown that the MMIP had high affinity and selectivity toward DA and other 
      structurally related CLs such as 3-methoxytyramine hydrochloride (MT), 
      DL-normetanephrine hydrochloride (NME), DL-norephinephrine hydrochloride (NE) and 
      (+/-) epinephrine (E). Different parameters affecting the extraction efficiency 
      were evaluated in order to achieve the optimal pre-concentration of the analytes 
      and to reduce non-specific interactions. Under the optimal conditions, the CL 
      limits of detection were at the 0.04-0.06 muM range. The relative standard 
      deviations of migration time and response ranged from 0.7% to 1.4% and from 2.9% 
      to 5.5%, respectively. The proposed method was successfully applied to determine 
      CLs, including MT, NME, DA, NE and E in human urine samples.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Bouri, Mohamed
AU  - Bouri M
AD  - Department of Analytical Chemistry and Food Technology, University of Castilla-La 
      Mancha, Av. Camilo Jose Cela 10, E-13004, Ciudad Real, Spain.
FAU - Lerma-Garcia, M Jesus
AU  - Lerma-Garcia MJ
FAU - Salghi, Rachid
AU  - Salghi R
FAU - Zougagh, Mohammed
AU  - Zougagh M
FAU - Rios, Angel
AU  - Rios A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120725
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Catecholamines)
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Methacrylates)
RN  - 0 (Polymers)
RN  - 1CS02G8656 (methacrylic acid)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Catecholamines/*isolation & purification/*urine
MH  - Dopamine/*chemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Humans
MH  - Magnetite Nanoparticles/chemistry
MH  - Magnets/*chemistry
MH  - Methacrylates/chemistry
MH  - Molecular Imprinting/*methods
MH  - Polymers/*chemical synthesis
MH  - Time Factors
EDAT- 2012/09/13 06:00
MHDA- 2013/02/13 06:00
CRDT- 2012/09/13 06:00
PHST- 2012/03/19 00:00 [received]
PHST- 2012/07/14 00:00 [revised]
PHST- 2012/07/19 00:00 [accepted]
PHST- 2012/09/13 06:00 [entrez]
PHST- 2012/09/13 06:00 [pubmed]
PHST- 2013/02/13 06:00 [medline]
AID - S0039-9140(12)00629-7 [pii]
AID - 10.1016/j.talanta.2012.07.053 [doi]
PST - ppublish
SO  - Talanta. 2012 Sep 15;99:897-903. doi: 10.1016/j.talanta.2012.07.053. Epub 2012 
      Jul 25.