PMID- 22967705
OWN - NLM
STAT- MEDLINE
DCOM- 20130619
LR  - 20151119
IS  - 1096-1194 (Electronic)
IS  - 0890-8508 (Linking)
VI  - 27
IP  - 1
DP  - 2013 Feb
TI  - Identification of potential biomarkers to distinguish Mycobacterium colombiense 
      from other mycobacterial species.
PG  - 46-52
LID - S0890-8508(12)00095-3 [pii]
LID - 10.1016/j.mcp.2012.08.009 [doi]
AB  - Mycobacterium avium complex (MAC) consists of 9 species of slow-growing 
      mycobacteria with differing degrees of pathogenicity, host preference and 
      environmental distribution. Mycobacterium colombiense is a novel member of MAC 
      that is responsible for disseminated infections in HIV-infected patients in 
      Colombia and lymphadenopathy cases in Europe. At present, methods to easily 
      differentiate novel members of MAC are lacking. In this study, we identified 
      possible biomarkers that are potentially useful for the detection of 
      M. colombiense by PCR or chromatography. The Randomly Amplified Polymorphic DNA 
      (RAPD) technique was used to amplify genomic fragments of M. colombiense CECT 
      3035 that were subsequently used in the development of a direct colony-specific 
      PCR assay using specific primers. The designed primers amplified a 634-bp 
      fragment of DNA from M. colombiense, which included a 450-bp genomic region that 
      encodes a hypothetical protein of 149 amino acids that is exclusive to 
      M. colombiense. Bioinformatic analyses revealed that this hypothetical protein 
      had no signal peptide, active sites or functional domains to aid its 
      identification or classification. In addition, using thin-layer chromatography, 
      we identified a different profile of mycolates for M. colombiense strains. The 
      test developed in this study has potential applications in the routine 
      identification of M. colombiense and in molecular assays designed for the 
      surveillance of MAC strains.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Leguizamon, John
AU  - Leguizamon J
AD  - Chemistry Department, Faculty of Sciences, Universidad Nacional de Colombia, 
      Carrera 30# 45-03, Ciudad Universitaria, Bogota, Colombia.
FAU - Hernandez, Johana
AU  - Hernandez J
FAU - Murcia, Martha-Isabel
AU  - Murcia MI
FAU - Soto, Carlos-Yesid
AU  - Soto CY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120829
PL  - England
TA  - Mol Cell Probes
JT  - Molecular and cellular probes
JID - 8709751
RN  - 0 (Bacterial Proteins)
RN  - 0 (Biomarkers)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacterial Proteins/chemistry/*genetics
MH  - Bacterial Typing Techniques
MH  - Base Sequence
MH  - Biomarkers
MH  - Chromatography, Thin Layer
MH  - DNA Primers/genetics
MH  - DNA, Bacterial/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mycobacterium avium Complex/*classification/*genetics
MH  - Mycobacterium avium-intracellulare Infection/diagnosis
MH  - Random Amplified Polymorphic DNA Technique
MH  - Sequence Analysis, DNA
EDAT- 2012/09/13 06:00
MHDA- 2013/06/20 06:00
CRDT- 2012/09/13 06:00
PHST- 2012/06/27 00:00 [received]
PHST- 2012/08/10 00:00 [revised]
PHST- 2012/08/17 00:00 [accepted]
PHST- 2012/09/13 06:00 [entrez]
PHST- 2012/09/13 06:00 [pubmed]
PHST- 2013/06/20 06:00 [medline]
AID - S0890-8508(12)00095-3 [pii]
AID - 10.1016/j.mcp.2012.08.009 [doi]
PST - ppublish
SO  - Mol Cell Probes. 2013 Feb;27(1):46-52. doi: 10.1016/j.mcp.2012.08.009. Epub 2012 
      Aug 29.