PMID- 22972023
OWN - NLM
STAT- MEDLINE
DCOM- 20130312
LR  - 20181202
IS  - 1748-605X (Electronic)
IS  - 1748-6041 (Linking)
VI  - 7
IP  - 5
DP  - 2012 Oct
TI  - Nanocomposites of high-density polyethylene with amorphous calcium phosphate: in 
      vitro biomineralization and cytocompatibility of human mesenchymal stem cells.
PG  - 054103
LID - 10.1088/1748-6041/7/5/054103 [doi]
AB  - Polyethylene is widely used as a component of implants in medicine. Composites 
      made of high-density polyethylene (HDPE) containing different amounts of 
      amorphous calcium phosphate nanoparticles were investigated concerning their in 
      vitro biomedical performance. The nanoparticles were produced by flame spray 
      synthesis and extruded with HDPE, the latter complying with Food and Drug 
      Administration regulations. Mechanical properties such as Young's modulus and 
      contact angle as well as in vitro biomineralization of the nanocomposites 
      hot-pressed into thin films were evaluated. The deposition of a hydroxyapatite 
      layer occurred upon immersion in simulated body fluid. Additionally, a cell 
      culture study with human mesenchymal stem cells for six weeks allowed a primary 
      assessment of the cytocompatibility. Viability assays (alamarBlue and lactate 
      dehydrogenase detection) proved the absence of cytotoxic effects of the 
      scaffolds. Microscopic images after hematoxylin and eosin staining confirmed 
      typical growth and morphology. A preliminary experiment analyzed the alkaline 
      phosphatase activity after two weeks. These findings motivate further 
      investigations on bioactive HDPE in bone tissue engineering.
FAU - Hild, Nora
AU  - Hild N
AD  - Department of Chemistry and Applied Biosciences, Institute for Chemical and 
      Bioengineering, ETH Zurich, 8093 Zurich, Switzerland.
FAU - Fuhrer, Roland
AU  - Fuhrer R
FAU - Mohn, Dirk
AU  - Mohn D
FAU - Bubenhofer, Stephanie B
AU  - Bubenhofer SB
FAU - Grass, Robert N
AU  - Grass RN
FAU - Luechinger, Norman A
AU  - Luechinger NA
FAU - Feldman, Kirill
AU  - Feldman K
FAU - Dora, Claudio
AU  - Dora C
FAU - Stark, Wendelin J
AU  - Stark WJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120912
PL  - England
TA  - Biomed Mater
JT  - Biomedical materials (Bristol, England)
JID - 101285195
RN  - 0 (Apatites)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Bone Substitutes)
RN  - 0 (Calcium Phosphates)
RN  - 0 (amorphous calcium phosphate)
RN  - 9002-88-4 (Polyethylene)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Apatites/chemistry
MH  - Biocompatible Materials/*chemistry
MH  - Body Fluids/chemistry
MH  - Bone Substitutes/chemistry
MH  - Calcium Phosphates/*chemistry
MH  - Cell Survival
MH  - Elastic Modulus
MH  - Humans
MH  - Materials Testing
MH  - Mesenchymal Stem Cells/*cytology/enzymology
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - Nanocomposites/*chemistry/ultrastructure
MH  - Polyethylene/*chemistry
MH  - Tissue Engineering
MH  - Tissue Scaffolds/chemistry
EDAT- 2012/09/14 06:00
MHDA- 2013/03/13 06:00
CRDT- 2012/09/14 06:00
PHST- 2012/09/14 06:00 [entrez]
PHST- 2012/09/14 06:00 [pubmed]
PHST- 2013/03/13 06:00 [medline]
AID - 10.1088/1748-6041/7/5/054103 [doi]
PST - ppublish
SO  - Biomed Mater. 2012 Oct;7(5):054103. doi: 10.1088/1748-6041/7/5/054103. Epub 2012 
      Sep 12.