PMID- 22974612
OWN - NLM
STAT- MEDLINE
DCOM- 20130211
LR  - 20191210
IS  - 1531-5037 (Electronic)
IS  - 0022-3468 (Linking)
VI  - 47
IP  - 9
DP  - 2012 Sep
TI  - Comparison of the protective effect of dipyridamole and acetylsalicylic acid on 
      long-term histologic damage in a rat model of testicular ischemia-reperfusion 
      injury.
PG  - 1716-23
LID - S0022-3468(12)00153-4 [pii]
LID - 10.1016/j.jpedsurg.2012.01.078 [doi]
AB  - PURPOSE: Ischemia reperfusion injury arising from testicular torsion results in a 
      loss of spermatogenesis and a significant increase in germ cell apoptosis. We 
      investigated the effects of dipyridamole and acetylsalicylic acid (ASA), 2 
      well-known platelet inhibitors, on testicular ischemia reperfusion injury. 
      METHODS: Thirty adult male Sprague-Dawley rats were randomly divided into 5 
      groups (n = 6 for each group): control, sham-operated, torsion/detorsion (T/D), 
      T/D + dipyridamole, and T/D + ASA. Testicular ischemia was achieved by rotating 
      the left testis 720 degrees  clockwise for 2 hours. Thirty minutes before torsion, 10 
      mg/kg dipyridamole was injected transperitoneally in the T/D + dipyridamole 
      group, and 100 mg/kg ASA was injected transperitoneally in the T/D + ASA group. 
      Sixty days after the initial surgical procedure, ipsilateral orchiectomies were 
      performed for histopathologic examination to determine Johnsen's mean testicular 
      biopsy score (MTBS), mean seminiferous tubular diameter (MSTD), and apoptotic 
      index (AI) in all groups. RESULTS: Unilateral testicular torsion-detorsion led to 
      a significant decrease in Johnsen's MTBS and MSTD values in the ipsilateral 
      testis and a significant increase in AI values of the T/D group. There were no 
      significant differences between the T/D + dipyridamole and control groups in 
      terms of MSTD and MTBS values. Although an amount of improvement exits in T/D + 
      ASA group, there were significant differences between the T/D + ASA and control 
      group MSTD and MTBS values. There was no significant difference between the T/D + 
      dipyridamole and control groups in terms of AI values (P > .05), but the 
      differences between the T/D + ASA and control groups were significant despite a 
      slight decline in AI values of the T/D + ASA group. CONCLUSIONS: Our findings 
      show that the use of dipyridamole before testicular reperfusion has a potentially 
      protective effect against long-term injury in testicular ischemia reperfusion 
      injury.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Karaguzel, Ersagun
AU  - Karaguzel E
AD  - Department of Urology, Karadeniz Technical University, School of Medicine, 61080 
      Trabzon, Turkey. ersagunkaraguzel@gmail.com
FAU - Kutlu, Omer
AU  - Kutlu O
FAU - Yulug, Esin
AU  - Yulug E
FAU - Mungan, Sevdegul
AU  - Mungan S
FAU - Kazaz, Ilke Onur
AU  - Kazaz IO
FAU - Tok, Dogan Sabri
AU  - Tok DS
FAU - Ozgur, Guner Kemal
AU  - Ozgur GK
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pediatr Surg
JT  - Journal of pediatric surgery
JID - 0052631
RN  - 0 (Platelet Aggregation Inhibitors)
RN  - 64ALC7F90C (Dipyridamole)
RN  - R16CO5Y76E (Aspirin)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Aspirin/pharmacology/*therapeutic use
MH  - Dipyridamole/pharmacology/*therapeutic use
MH  - Disease Models, Animal
MH  - Drug Administration Schedule
MH  - In Situ Nick-End Labeling
MH  - Injections, Intraperitoneal
MH  - Male
MH  - Platelet Aggregation Inhibitors/pharmacology/*therapeutic use
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/etiology/pathology/*prevention & control
MH  - Spermatic Cord Torsion/*complications/pathology
MH  - Spermatozoa/drug effects/pathology
MH  - Testis/drug effects/pathology
MH  - Treatment Outcome
EDAT- 2012/09/15 06:00
MHDA- 2013/02/12 06:00
CRDT- 2012/09/15 06:00
PHST- 2011/12/01 00:00 [received]
PHST- 2012/01/05 00:00 [revised]
PHST- 2012/01/31 00:00 [accepted]
PHST- 2012/09/15 06:00 [entrez]
PHST- 2012/09/15 06:00 [pubmed]
PHST- 2013/02/12 06:00 [medline]
AID - S0022-3468(12)00153-4 [pii]
AID - 10.1016/j.jpedsurg.2012.01.078 [doi]
PST - ppublish
SO  - J Pediatr Surg. 2012 Sep;47(9):1716-23. doi: 10.1016/j.jpedsurg.2012.01.078.