PMID- 22975347
OWN - NLM
STAT- MEDLINE
DCOM- 20130227
LR  - 20220408
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 427
IP  - 1
DP  - 2012 Oct 12
TI  - Co-culture with Sertoli cells promotes proliferation and migration of umbilical 
      cord mesenchymal stem cells.
PG  - 86-90
LID - S0006-291X(12)01734-2 [pii]
LID - 10.1016/j.bbrc.2012.09.007 [doi]
AB  - Human umbilical cord mesenchymal stem cells (hUCMSCs) have been recently used in 
      transplant therapy. The proliferation and migration of MSCs are the determinants 
      of the efficiency of MSC transplant therapy. Sertoli cells are a kind of "nurse" 
      cells that support the development of sperm cells. Recent studies show that 
      Sertoli cells promote proliferation of endothelial cells and neural stem cells in 
      co-culture. We hypothesized that co-culture of UCMSCs with Sertoli cells may also 
      promote proliferation and migration of UCMSCs. To examine this hypothesis, we 
      isolated UCMSCs from human cords and Sertoli cells from mouse testes, and 
      co-cultured them using a Transwell system. We found that UCMSCs exhibited strong 
      proliferation ability and potential to differentiate to other cell lineages such 
      as osteocytes and adipocytes. The presence of Sertoli cells in co-culture 
      significantly enhanced the proliferation and migration potential of UCMSCs 
      (P<0.01). Moreover, these phenotypic changes were accompanied with upregulation 
      of multiple genes involved in cell proliferation and migration including 
      phospho-Akt, Mdm2, phospho-CDC2, Cyclin D1, Cyclin D3 as well as CXCR4, 
      phospho-p44 MAPK and phospho-p38 MAPK. These findings indicate that Sertoli cells 
      boost UCMSC proliferation and migration potential.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Zhang, Fenxi
AU  - Zhang F
AD  - Department of Anatomy, Sanquan College, Xinxiang Medical University, Henan 
      453003, People's Republic of China. fxzhang0824@gmail.com
FAU - Hong, Yan
AU  - Hong Y
FAU - Liang, Wenmei
AU  - Liang W
FAU - Ren, Tongming
AU  - Ren T
FAU - Jing, Suhua
AU  - Jing S
FAU - Lin, Juntang
AU  - Lin J
LA  - eng
PT  - Journal Article
DEP - 20120910
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
SB  - IM
MH  - Adipocytes/cytology
MH  - Animals
MH  - Cell Differentiation
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - Coculture Techniques
MH  - Female
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*physiology
MH  - Mice
MH  - Osteoblasts/cytology
MH  - Sertoli Cells/*physiology
MH  - Umbilical Cord/*cytology
EDAT- 2012/09/15 06:00
MHDA- 2013/02/28 06:00
CRDT- 2012/09/15 06:00
PHST- 2012/08/27 00:00 [received]
PHST- 2012/09/01 00:00 [accepted]
PHST- 2012/09/15 06:00 [entrez]
PHST- 2012/09/15 06:00 [pubmed]
PHST- 2013/02/28 06:00 [medline]
AID - S0006-291X(12)01734-2 [pii]
AID - 10.1016/j.bbrc.2012.09.007 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 Oct 12;427(1):86-90. doi: 
      10.1016/j.bbrc.2012.09.007. Epub 2012 Sep 10.