PMID- 22978418
OWN - NLM
STAT- MEDLINE
DCOM- 20130418
LR  - 20121030
IS  - 1365-2559 (Electronic)
IS  - 0309-0167 (Linking)
VI  - 61
IP  - 5
DP  - 2012 Nov
TI  - Atopic and non-atopic eosinophilic oesophagitis are distinguished by 
      immunoglobulin E-bearing intraepithelial mast cells.
PG  - 810-22
LID - 10.1111/j.1365-2559.2012.4303.x [doi]
AB  - AIMS: Eosinophilic oesophagitis (EoE) occurs in atopic individuals and features 
      eosinophils and mast cells, but differences in the inflammatory cell density 
      between the epithelium and lamina propria (LP) are not fully understood. The aim 
      of this study was to determine if numbers of eosinophils, B lymphocytes and 
      immunoglobulin E (IgE)-bearing mast cells are increased in the mucosa of EoE 
      patients with and without concurrent atopy. METHODS AND RESULTS: Oesophageal 
      biopsies containing >/= 4 high-power fields (HPF) of epithelium and LP were 
      identified for normal (n = 9), gastroesophageal reflux disease (GERD) (n = 5) and 
      EoE (n = 25) patients. Patients were classified as atopic or not by clinical 
      history. Immunohistochemistry identified mast cells, B lymphocytes and 
      eosinophils. Eosinophil density was increased in the LP in EoE. Intraepithelial 
      eosinophil density correlated with eosinophils/HPF, CD20(+) B lymphocyte density 
      and tryptase(+) IgE(+) mast cell density. Increased intraepithelial IgE(+) cell 
      density in EoE was associated with mast cells and not B lymphocytes. 
      Intraepithelial IgE(+) mast cell densities were significantly higher in biopsies 
      from the subgroup of EoE patients with atopy. CONCLUSIONS: EoE diagnosis using 
      maximal eosinophil count/HPF correlates with average counts/mm(2), and 
      intraepithelial eosinophil densities are higher in children than adults with EoE. 
      In EoE, numbers of eosinophils and mast cells are increased in the LP. 
      IgE-bearing mast cells are increased in atopic EoE patients but not in non-atopic 
      EoE patients.
CI  - (c) 2012 Blackwell Publishing Limited.
FAU - Mulder, Daniel J
AU  - Mulder DJ
AD  - Department of Anatomy and Cell Biology, Gastrointestinal Diseases Research Unit, 
      Queen's University, Kingston, Ontario, Canada.
FAU - Mak, Nanette
AU  - Mak N
FAU - Hurlbut, David J
AU  - Hurlbut DJ
FAU - Justinich, Christopher J
AU  - Justinich CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120917
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - B-Lymphocytes/pathology
MH  - Cell Count
MH  - Child
MH  - Child, Preschool
MH  - Eosinophilic Esophagitis/*immunology/*pathology
MH  - Eosinophils/pathology
MH  - Epithelial Cells/immunology/pathology
MH  - Female
MH  - Gastroesophageal Reflux/immunology/pathology
MH  - Humans
MH  - Hypersensitivity, Immediate/*immunology/*pathology
MH  - Immunoglobulin E/*metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Mast Cells/*immunology/*pathology
MH  - Middle Aged
MH  - Mucous Membrane/immunology/pathology
EDAT- 2012/09/18 06:00
MHDA- 2013/04/20 06:00
CRDT- 2012/09/18 06:00
PHST- 2012/09/18 06:00 [entrez]
PHST- 2012/09/18 06:00 [pubmed]
PHST- 2013/04/20 06:00 [medline]
AID - 10.1111/j.1365-2559.2012.4303.x [doi]
PST - ppublish
SO  - Histopathology. 2012 Nov;61(5):810-22. doi: 10.1111/j.1365-2559.2012.4303.x. Epub 
      2012 Sep 17.