PMID- 22978816
OWN - NLM
STAT- MEDLINE
DCOM- 20130213
LR  - 20211021
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 84
IP  - 19
DP  - 2012 Oct 2
TI  - Detection of single influenza viral RNA in cells using a polymeric sequence 
      probe.
PG  - 8118-21
LID - 10.1021/ac3023873 [doi]
AB  - A polynucleotide probe, call a polymeric sequence probe (PSP), was used to detect 
      influenza A (Influenza A/WSN/33) NA (Neuraminidase) viral RNA in Madin-Darby 
      canine kidney (MDCK) cells. The PSP is a single-stranded DNA molecule with ~2,000 
      tandem repeat fluorescence binding sites and target binding sites that can bind 
      with multiple fluorescence complementary oligos and target viral RNA using a 
      fluorescence in situ hybridization (FISH) process. A single viral RNA labeled by 
      PSP can be directly observed in MDCK cells. The simple FISH protocol enables the 
      observation and quantitative analysis of the infectious process and drug effects 
      with ultrahigh sensitivity and spatial resolution.
FAU - Huang, Suxian
AU  - Huang S
AD  - California NanoSystems Institute, University of California, Los Angeles, Los 
      Angeles, California 90095, USA.
FAU - Yu, Chaoran
AU  - Yu C
FAU - Cheng, Genhong
AU  - Cheng G
FAU - Chen, Yong
AU  - Chen Y
LA  - eng
GR  - R01 AI069120/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120919
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Polynucleotides)
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Dogs
MH  - In Situ Hybridization, Fluorescence
MH  - Influenza A virus/*chemistry/*genetics
MH  - Madin Darby Canine Kidney Cells
MH  - Polynucleotides/*genetics
MH  - RNA Probes/*chemistry/*genetics
MH  - RNA, Viral/*analysis/*genetics
PMC - PMC4111224
MID - NIHMS583728
EDAT- 2012/09/18 06:00
MHDA- 2013/02/14 06:00
PMCR- 2014/07/25
CRDT- 2012/09/18 06:00
PHST- 2012/09/18 06:00 [entrez]
PHST- 2012/09/18 06:00 [pubmed]
PHST- 2013/02/14 06:00 [medline]
PHST- 2014/07/25 00:00 [pmc-release]
AID - 10.1021/ac3023873 [doi]
PST - ppublish
SO  - Anal Chem. 2012 Oct 2;84(19):8118-21. doi: 10.1021/ac3023873. Epub 2012 Sep 19.