PMID- 22979950
OWN - NLM
STAT- MEDLINE
DCOM- 20130226
LR  - 20211021
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 9
DP  - 2012 Sep 14
TI  - Identification of B cell epitopes reactive to human papillomavirus type-16L1- 
      derived peptides.
PG  - 199
LID - 10.1186/1743-422X-9-199 [doi]
AB  - BACKGROUND: Persistent infection of human papillomavirus (HPV) types 16 and 18 
      causes cervical cancer. To better understand immune responses to the prophylactic 
      vaccine, HPV 16/18 L1 virus-like particles (HPV-VLPs), we investigated B cell 
      epitopes of HPV16 L1-derived peptides. METHODS: Sera from mice immunized with 
      HPV-16/18 L1 VLPs were analyzed for their IgG titers against 10 different HPV16 
      L1-derived peptides (20-mer) that contain human leukocyte antigen (HLA)-class I 
      A-2, A-24 and class II DR. RESULTS: One 20-mer peptide at positions 300 to 319 
      was identified as a common B cell epitope in both Balb/c (H-2d) and C57BL/6 
      (H-2b) mice. Mapping analysis showed that the 10-amino-acid sequence at positions 
      304 to 313 was an immunogenic portion. It is of note that the binding capability 
      of this 10-mer peptide to the HLA-A2 and HLA-A24 molecules was confirmed by the 
      HLA class I stabilization assay. In addition, one unique 20-mer was determined as 
      a B cell epitope in each strain. CONCLUSIONS: These results might provide new 
      information for better understanding of immune responses to HPV 16 L1.
FAU - Fukui, Akimasa
AU  - Fukui A
AD  - Department of Obstetrics and Gynecology, Kurume University School of Medicine, 
      Kurume, Japan.
FAU - Matsueda, Satoko
AU  - Matsueda S
FAU - Kawano, Kouichiro
AU  - Kawano K
FAU - Tsuda, Naotake
AU  - Tsuda N
FAU - Komatsu, Nobukazu
AU  - Komatsu N
FAU - Shichijo, Shigeki
AU  - Shichijo S
FAU - Sasada, Tetsuro
AU  - Sasada T
FAU - Hattori, Satoshi
AU  - Hattori S
FAU - Ushijima, Kimio
AU  - Ushijima K
FAU - Itoh, Kyogo
AU  - Itoh K
FAU - Kamura, Toshiharu
AU  - Kamura T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120914
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (Antibodies, Viral)
RN  - 0 (Capsid Proteins)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (HLA-A Antigens)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 6LTE2DNX63 (L1 protein, Human papillomavirus type 16)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Capsid Proteins/*immunology
MH  - Epitope Mapping
MH  - Epitopes, B-Lymphocyte/*immunology
MH  - Female
MH  - HLA-A Antigens/metabolism
MH  - Humans
MH  - Immunoglobulin G/blood
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Oncogene Proteins, Viral/*immunology
MH  - Protein Binding
PMC - PMC3463485
EDAT- 2012/09/18 06:00
MHDA- 2013/02/27 06:00
PMCR- 2012/09/14
CRDT- 2012/09/18 06:00
PHST- 2012/02/22 00:00 [received]
PHST- 2012/09/11 00:00 [accepted]
PHST- 2012/09/18 06:00 [entrez]
PHST- 2012/09/18 06:00 [pubmed]
PHST- 2013/02/27 06:00 [medline]
PHST- 2012/09/14 00:00 [pmc-release]
AID - 1743-422X-9-199 [pii]
AID - 10.1186/1743-422X-9-199 [doi]
PST - epublish
SO  - Virol J. 2012 Sep 14;9:199. doi: 10.1186/1743-422X-9-199.