PMID- 22981434
OWN - NLM
STAT- MEDLINE
DCOM- 20130110
LR  - 20121008
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 433
IP  - 2
DP  - 2012 Nov 25
TI  - Dissociation of porcine reproductive and respiratory syndrome virus 
      neutralization from antibodies specific to major envelope protein surface 
      epitopes.
PG  - 367-76
LID - S0042-6822(12)00407-2 [pii]
LID - 10.1016/j.virol.2012.08.026 [doi]
AB  - Glycoprotein 5 (GP5) and membrane (M) protein are the major proteins in the 
      envelope of porcine reproductive and respiratory syndrome virus (PRRSV). Although 
      viral neutralization epitopes are reported in GP5 and M of type 2 PRRSV, their 
      significance as targets of porcine humoral immunity is not well described. Thus, 
      we constructed recombinant polypeptides containing ectodomain neutralization 
      epitopes to examine their involvement in porcine antibody neutralization and 
      antiviral immunity. PRRSV infection elicited ectodomain-specific antibodies, 
      whose titers did not correlate with the neutralizing antibody (NA) response. 
      Ectodomain-specific antibodies from PRRSV-neutralizing serum bound virus but did 
      not neutralize infectivity. Furthermore, immunization of pigs with ectodomain 
      polypeptides raised specific antibodies and provided partial protection without a 
      detectable NA response. Finally the polypeptides did not block infection of 
      porcine macrophages. These results suggest that the GP5/M ectodomain peptide 
      epitopes are accessible for host antibody recognition, but are not associated 
      with antibody-mediated virus neutralization.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Li, Juan
AU  - Li J
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota, St. 
      Paul, MN 55108, USA.
FAU - Murtaugh, Michael P
AU  - Murtaugh MP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120912
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Epitopes)
RN  - 0 (M protein, porcine reproductive and respiratory syndrome virus)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Neutralizing/biosynthesis
MH  - Antibodies, Viral/biosynthesis
MH  - Antibody Affinity
MH  - Antibody Specificity
MH  - Antigens, Viral/genetics
MH  - Epitopes/genetics
MH  - Female
MH  - Immunization
MH  - Macrophages, Alveolar/immunology/virology
MH  - Male
MH  - Molecular Sequence Data
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/*immunology/pathogenicity
MH  - Sus scrofa
MH  - Swine
MH  - Viral Envelope Proteins/genetics/immunology
MH  - Viral Matrix Proteins/genetics/immunology
EDAT- 2012/09/18 06:00
MHDA- 2013/01/11 06:00
CRDT- 2012/09/18 06:00
PHST- 2012/06/02 00:00 [received]
PHST- 2012/06/09 00:00 [revised]
PHST- 2012/08/17 00:00 [accepted]
PHST- 2012/09/18 06:00 [entrez]
PHST- 2012/09/18 06:00 [pubmed]
PHST- 2013/01/11 06:00 [medline]
AID - S0042-6822(12)00407-2 [pii]
AID - 10.1016/j.virol.2012.08.026 [doi]
PST - ppublish
SO  - Virology. 2012 Nov 25;433(2):367-76. doi: 10.1016/j.virol.2012.08.026. Epub 2012 
      Sep 12.