PMID- 22982140
OWN - NLM
STAT- MEDLINE
DCOM- 20130529
LR  - 20181202
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 54
DP  - 2013 Jan
TI  - Essential role of toll-like receptor 4 in Acinetobacter baumannii-induced immune 
      responses in immune cells.
PG  - 20-5
LID - S0882-4010(12)00160-X [pii]
LID - 10.1016/j.micpath.2012.08.008 [doi]
AB  - TLR4 is a membrane sensor for lipopolysaccharide (LPS), a major cell wall 
      component of gram-negative bacteria. In this study, we investigated the role of 
      TLR4 on innate immune responses in immune cells against Acinetobacter baumannii. 
      Bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs) were isolated 
      from WT and TLR4-deficient mice and infected with A. baumannii ATCC 15150. ELISA 
      assay revealed that the production of IL-6 and TNF-alpha by A. baumannii was impaired 
      in TLR4-deficient macrophages. However, absence of TLR2 did not affect A. 
      baumannii-induced cytokines production in BMDMs. In addition, TLR4 was required 
      for the optimal production of IL-6, TNF-alpha, and IL-12 in BMDCs in response to A. 
      baumannii. Western blot analysis showed that A. baumannii leads to the activation 
      of NF-kappaB and MAPKs (p38, ERK, and JNK) in macrophages via TLR4-dependent pathway. 
      mRNA expression of iNOS and NO production was elicited in WT BMDMs in response to 
      A. baumannii, which was abolished in TLR4-deficienct cells. Bacterial killing 
      ability against A. baumannii was impaired in TLR4-deficient BMDMs. In addition, 
      A. baumannii induced apoptosis in BMDMs via TLR4-independent pathway. Our results 
      demonstrate that TLR4 is essential for initiating innate immune response of 
      macrophages against A. baumannii infection.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Kim, Chang-Hwan
AU  - Kim CH
AD  - Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul 
      National University, Gwanak-ro 1, Gwanak-gu, Seoul 151-742, Republic of Korea.
FAU - Jeong, Yu-Jin
AU  - Jeong YJ
FAU - Lee, Junglim
AU  - Lee J
FAU - Jeon, Soo-Jin
AU  - Jeon SJ
FAU - Park, Se-Ra
AU  - Park SR
FAU - Kang, Min-Jung
AU  - Kang MJ
FAU - Park, Jae-Hak
AU  - Park JH
FAU - Park, Jong-Hwan
AU  - Park JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120912
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 187348-17-0 (Interleukin-12)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - Acinetobacter baumannii/*immunology
MH  - Animals
MH  - Apoptosis
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/microbiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression Profiling
MH  - Interleukin-12/metabolism
MH  - Interleukin-6/metabolism
MH  - Macrophages/*immunology/microbiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mitogen-Activated Protein Kinase Kinases/biosynthesis
MH  - NF-kappa B/biosynthesis
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type II/biosynthesis
MH  - Toll-Like Receptor 4/deficiency/*immunology
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2012/09/18 06:00
MHDA- 2013/05/31 06:00
CRDT- 2012/09/18 06:00
PHST- 2012/05/17 00:00 [received]
PHST- 2012/08/28 00:00 [revised]
PHST- 2012/08/29 00:00 [accepted]
PHST- 2012/09/18 06:00 [entrez]
PHST- 2012/09/18 06:00 [pubmed]
PHST- 2013/05/31 06:00 [medline]
AID - S0882-4010(12)00160-X [pii]
AID - 10.1016/j.micpath.2012.08.008 [doi]
PST - ppublish
SO  - Microb Pathog. 2013 Jan;54:20-5. doi: 10.1016/j.micpath.2012.08.008. Epub 2012 
      Sep 12.