PMID- 22982892
OWN - NLM
STAT- MEDLINE
DCOM- 20121203
LR  - 20191210
IS  - 1532-0979 (Electronic)
IS  - 0147-5185 (Linking)
VI  - 36
IP  - 10
DP  - 2012 Oct
TI  - Evaluation of reliability of FISH versus brightfield dual-probe in situ 
      hybridization (BDISH) for frontline assessment of HER2 status in breast cancer 
      samples in a community setting: influence of poor tissue preservation.
PG  - 1489-96
LID - 10.1097/PAS.0b013e3182635987 [doi]
AB  - AIMS: To evaluate the reliability of novel brightfield microscopy-based dual in 
      situ hybridization (BDISH) methods for frontline HER2 status analysis in selected 
      suboptimally preserved breast cancer tissue samples reflecting of the worst 
      scenario in a community. METHODS AND RESULTS: A total of 320 morphologically 
      poorly preserved breast invasive ductal carcinomas from the archives of 2 
      tertiary institutions in Brazil were selected for a tissue microarray-based 
      analysis. 4B5 antibody was used for immunohistochemistry. Fluorescence in situ 
      hybridization (FISH), DuoCISH, ZytoDot CISH, and silver in situ hybridization 
      (SISH) were performed and compared. The highest agreement was observed between 
      SISH and FISH. In addition, SISH was easier to assess in both amplified and 
      nonamplified cases when compared with the other chromogenic methods, due to the 
      sharpness of its dots. DuoCISH produced false-positive results, associated with 
      thicker ill-defined dots, causing poor distinction between nonamplification and 
      low amplification. ZytoDot CISH showed lower sensitivity, with increased 
      frequency of false-positive results. CONCLUSIONS: SISH is the most reliable of 
      the BDISH methods, with sensitivity and specificity highly comparable with FISH. 
      It is also less deleterious than other BDISH methods, producing signals that were 
      more distinct and therefore more readily analyzable even in poorly preserved 
      tissue.
FAU - Schiavon, Beatriz N
AU  - Schiavon BN
AD  - Department of Anatomic Pathology, Hospital AC Camargo, Sao Paulo, SP, Brazil. 
      beatriznschi@gmail.com
FAU - Jasani, Bharat
AU  - Jasani B
FAU - de Brot, Louise
AU  - de Brot L
FAU - Vassallo, Jose
AU  - Vassallo J
FAU - Damascena, Aline
AU  - Damascena A
FAU - Cirullo-Neto, Julio
AU  - Cirullo-Neto J
FAU - Ivanildo Neves, Jose
AU  - Ivanildo Neves J
FAU - Augusto Soares, Fernando
AU  - Augusto Soares F
FAU - Gobbi, Helenice
AU  - Gobbi H
FAU - Malagoli Rocha, Rafael
AU  - Malagoli Rocha R
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Artifacts
MH  - Breast Neoplasms/diagnosis/*genetics
MH  - Carcinoma, Ductal, Breast/diagnosis/*genetics
MH  - Community Health Services
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Receptor, ErbB-2/*genetics
MH  - Reproducibility of Results
MH  - Tertiary Care Centers
MH  - Tissue Array Analysis
MH  - Tissue Preservation/*methods
EDAT- 2012/09/18 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/09/18 06:00
PHST- 2012/09/18 06:00 [entrez]
PHST- 2012/09/18 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 00000478-201210000-00009 [pii]
AID - 10.1097/PAS.0b013e3182635987 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2012 Oct;36(10):1489-96. doi: 10.1097/PAS.0b013e3182635987.