PMID- 22983155
OWN - NLM
STAT- MEDLINE
DCOM- 20130226
LR  - 20130109
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 158
IP  - 1
DP  - 2013 Jan
TI  - Combined 5' UTR RFLP analysis and VP1 sequencing for epidemic investigation of 
      enteroviruses.
PG  - 103-11
LID - 10.1007/s00705-012-1472-y [doi]
AB  - Enteroviruses, the main cause of aseptic meningitis, consist of 100 serotypes, 
      and many of them have been associated with large outbreaks. In the present study, 
      a comparison of RFLP analysis of the 5' untranslated region (5'UTR) and 
      sequencing of both the 5'UTR and VP1 regions was conducted for epidemiological 
      linkage of 27 clinical enterovirus strains. The clinical enterovirus strains were 
      clustered into five restriction profile groups. Even though the restriction 
      profile clusters of clinical isolates were not related to those of the respective 
      prototype strains, epidemiological relationships between the members of each 
      cluster were observed. The restriction profile clusters in the 5'UTR corresponded 
      to the phylogenetic clusters in the VP1 genomic region. The incongruence between 
      the topology of Gior strain in 5'UTR and VP1 phylogenetic trees indicates a 
      recombination event. The proposed RFLP assay in combination with VP1 sequencing 
      can offer crucial epidemiological information about the circulating 
      enteroviruses.
FAU - Kyriakopoulou, Zaharoula
AU  - Kyriakopoulou Z
AD  - Department of Biochemistry and Biotechnology, University of Thessaly, 26, 
      Ploutonos & Aeolou str., Larissa 41221, Greece.
FAU - Tsolis, Kostas
AU  - Tsolis K
FAU - Pliaka, Vaia
AU  - Pliaka V
FAU - Tsakogiannis, Dimitris
AU  - Tsakogiannis D
FAU - Ruether, Irina Georgia Anna
AU  - Ruether IG
FAU - Gartzonika, Constantina
AU  - Gartzonika C
FAU - Levidiotou-Stefanou, Stamatina
AU  - Levidiotou-Stefanou S
FAU - Markoulatos, Panayotis
AU  - Markoulatos P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120916
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Enterovirus/classification/*genetics/isolation & purification
MH  - Enterovirus Infections/epidemiology/*virology
MH  - Epidemics
MH  - Greece/epidemiology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - *Polymorphism, Restriction Fragment Length
MH  - Viral Envelope Proteins/*genetics
EDAT- 2012/09/18 06:00
MHDA- 2013/02/27 06:00
CRDT- 2012/09/18 06:00
PHST- 2012/04/04 00:00 [received]
PHST- 2012/07/30 00:00 [accepted]
PHST- 2012/09/18 06:00 [entrez]
PHST- 2012/09/18 06:00 [pubmed]
PHST- 2013/02/27 06:00 [medline]
AID - 10.1007/s00705-012-1472-y [doi]
PST - ppublish
SO  - Arch Virol. 2013 Jan;158(1):103-11. doi: 10.1007/s00705-012-1472-y. Epub 2012 Sep 
      16.