PMID- 22984623 OWN - NLM STAT- MEDLINE DCOM- 20130305 LR - 20211203 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 7 IP - 9 DP - 2012 TI - The mTOR inhibitor rapamycin has limited acute anticonvulsant effects in mice. PG - e45156 LID - 10.1371/journal.pone.0045156 [doi] LID - e45156 AB - OBJECTIVE: The mammalian target of rapamycin (mTOR) pathway integrates signals from different nutrient sources, including amino acids and glucose. Compounds that inhibit mTOR kinase activity such as rapamycin and everolimus can suppress seizures in some chronic animal models and in patients with tuberous sclerosis. However, it is not known whether mTOR inhibitors exert acute anticonvulsant effects in addition to their longer term antiepileptogenic effects. To gain insights into how rapamycin suppresses seizures, we investigated the anticonvulsant activity of rapamycin using acute seizure tests in mice. METHODS: Following intraperitoneal injection of rapamycin, normal four-week-old male NIH Swiss mice were evaluated for susceptibility to a battery of acute seizure tests similar to those currently used to screen potential therapeutics by the US NIH Anticonvulsant Screening Program. To assess the short term effects of rapamycin, mice were seizure tested in